Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit | MMP2 elisa kit

Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit

Cat. Num. AAA7606530

• Gene Names: Mmp2; 2-MMP; anon-WO0118547.84; CG1794; dm-2MMP; Dm2-MMP; DmelCG1794; dmmp2; l(2)02353; mmp2; MMP2
• Reactivity: Canine
• Synonyms: MMP2 (Matrix metalloproteinase-2); Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; CLG4A; MMP2 elisa kit

• Reactivity: Canine
• Sequence Length: 650

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MMP2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 442623038
• NCBI GeneID: 35997
• NCBI Accession #: NP_610511.3
• NCBI GenBank Nucleotide #: NM_136667.3
• Molecular Weight: 77,261 Da
• NCBI Official Full Name: matrix metalloproteinase 2, isoform D
• NCBI Official Synonym Full Names: Matrix metalloproteinase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: 2-MMP; anon-WO0118547.84; CG1794; dm-2MMP; Dm2-MMP; DmelCG1794; dmmp2; l(2)02353; mmp2; MMP2
• NCBI Protein Information: CG1794 gene product from transcript CG1794-RB
• UniProt Protein Name: Matrix metalloproteinase-2
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: Dm2-MMP

Uniprot Description

Has metalloproteinase activity (PubMed:11967260). Required for larval tissue histolysis during metamorphosis and is involved in pupal head eversion and fusion of the wing imaginal tissue (PubMed:12530966). Required for growth of the dorsal air sac primordium and development of the dorsal air sacs (PubMed:19751719). Promotes embryonic motor axon fasciculation (PubMed:18045838). Cleaves and activates frac to promote motor axon bundling during outgrowth (PubMed:21471368). Promotes the reshaping of adult sensory neuron dendrites from a radial to lattice-like shape which occurs after eclosion by degrading the basement membrane on which the dendrites grow (PubMed:20412776). Involved in inhibition of follicle stem cell proliferation by cleaving Dlp, inhibiting its interaction with wg and preventing Dlp-mediated spreading of wg to follicle stem cells to enhance their proliferation (PubMed:25267296). Plays a role in wound healing (PubMed:22262460). Involved in fat body dissociation which occurs during metamorphosis by degrading basement membrane components, leading to destruction of cell-basement membrane junctions (PubMed:25520167). Required for posterior follicle cell degradation and ovulation (PubMed:25695427).