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Western Blot (WB) (Western blot analysis of MAT2B Antibody (N-term) in293,MDA-MB231 cell line lysates (35ug/lane). MAT2B (arrow) was detected using the purified Pab.)

Rabbit anti-Human MAT2B Polyclonal Antibody | anti-MAT2B antibody

MAT2B, NT (MAT2B, TGR, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase) (AP)

Gene Names
MAT2B; TGR; MAT-II; SDR23E1; MATIIbeta; Nbla02999
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MAT2B; Polyclonal Antibody; NT (MAT2B; TGR; Methionine adenosyltransferase 2 subunit beta; Methionine adenosyltransferase II beta; Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase) (AP); Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase; anti-MAT2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-MAT2B antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MAT2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86~116 amino acids from the N-terminal region of human MAT2B.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAT2B Antibody (N-term) in293,MDA-MB231 cell line lysates (35ug/lane). MAT2B (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of MAT2B Antibody (N-term) in293,MDA-MB231 cell line lysates (35ug/lane). MAT2B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MAT2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MAT2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-MAT2B antibody
MAT2B belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT.
Product Categories/Family for anti-MAT2B antibody
References
Martinez, C.A., et al. Am. J. Obstet. Gynecol. 201 (4), 394 (2009): Boyles, A.L., et al. Genet. Epidemiol. 33(3):247-255(2009). Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009): Attia, R.R., et al. J. Biol. Chem. 283(45):30788-30795(2008). Wang, Q., et al. World J. Gastroenterol. 14(29):4633-4642(2008). LeGros, L., et al. J. Biol. Chem. 276(27):24918-24924(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,488 Da
NCBI Official Full Name
methionine adenosyltransferase 2 subunit beta isoform 1
NCBI Official Synonym Full Names
methionine adenosyltransferase II, beta
NCBI Official Symbol
MAT2B
NCBI Official Synonym Symbols
TGR; MAT-II; SDR23E1; MATIIbeta; Nbla02999
NCBI Protein Information
methionine adenosyltransferase 2 subunit beta; MAT II beta; beta regulatory subunit of methionine adenosyltransferase; dTDP-4-keto-6-deoxy-D-glucose 4-reductase; putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase; putative protein product of Nbla02999; sh
UniProt Protein Name
Methionine adenosyltransferase 2 subunit beta
UniProt Gene Name
MAT2B
UniProt Synonym Gene Names
TGR; MAT II beta
UniProt Entry Name
MAT2B_HUMAN

Uniprot Description

MAT2B: Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S- adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S- adenosylmethionine inhibition. Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: methionine adenosyltransferase complex; mitochondrion; intracellular; cytosol; nucleus

Molecular Function: enzyme binding; methionine adenosyltransferase regulator activity

Biological Process: methylation; regulation of catalytic activity; xenobiotic metabolic process; S-adenosylmethionine biosynthetic process; one-carbon compound metabolic process

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Product Notes

The MAT2B mat2b (Catalog #AAA6319166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAT2B, NT (MAT2B, TGR, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAT2B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAT2B mat2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAT2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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