<
Western Blot (WB)
(Anti- MASPIN Picoband antibody, MBS178287, Western blottingAll lanes: Anti MASPIN (MBS178287) at 0.5ug/mlLane 1: Mouse Intestine Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
MASPIN Polyclonal Antibody | anti-MASPIN antibody
Anti-MASPIN Antibody
Gene Names
SERPINB5; PI5; maspin
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen Affinity Purified
Synonyms
MASPIN; Polyclonal Antibody; Anti-MASPIN Antibody; Serpin B5; mammary serine proteinase inhibitor; Maspin; Ovalbumin; Peptidase inhibitor 5; PI-5; PI5; Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor; clade B; member 5; serpin peptidase inhibitor; Serpin peptidase inhibitor; clade B (ovalbumin); SERPINB5; SPB5_HUMAN; Spi5; Spi7; anti-MASPIN antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
375
Applicable Applications for anti-MASPIN antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunocytochemistry Concentration: 0.5-1ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunocytochemistry Concentration: 0.5-1ug/ml
Immunogen
E Coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- MASPIN Picoband antibody, MBS178287, Western blottingAll lanes: Anti MASPIN (MBS178287) at 0.5ug/mlLane 1: Mouse Intestine Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Western Blot (WB)
(Anti- MASPIN Picoband antibody, MBS178287, Western blottingAll lanes: Anti MASPIN (MBS178287) at 0.5ug/mlLane 1: Mouse Intestine Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: MM231 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Mouse Intestine Tissue )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Mouse Intestine Tissue )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Rat Intestine Tissue )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti- MASPIN Picoband antibody, MBS178287, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunocytochemistry (ICC)
(Anti- MASPIN Picoband antibody, MBS178287, ICCICC: MCF-7 Cell )
Immunocytochemistry (ICC)
(Anti- MASPIN Picoband antibody, MBS178287, ICCICC: MCF-7 Cell )
Related Product Information for anti-MASPIN antibody
Description: Rabbit IgG polyclonal antibody for Serpin B5(SERPINB5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Background: SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
Background: SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
References
1. Blandamura S, et al. Maspin expression, subcellular localization and clinicopathological correlation in endometrial hyperplasia and endometrial adenocarcinoma. Histol Histopathol, 2014 Jun. 2. Taskiran C, et al. Maspin expression in endometrial hyperplasia and carcinoma, and its relation with angiogenesis. Eur J Gynaecol Oncol, 2014. 3. Triulzi T, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer, 2014 Jun 15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,763 Da
NCBI Official Full Name
serpin B5
NCBI Official Synonym Full Names
serpin family B member 5
NCBI Official Symbol
SERPINB5
NCBI Official Synonym Symbols
PI5; maspin
NCBI Protein Information
serpin B5
UniProt Protein Name
Serpin B5
UniProt Gene Name
SERPINB5
UniProt Synonym Gene Names
PI5; PI-5
UniProt Entry Name
SPB5_HUMAN
Uniprot Description
maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 18q21.33
Cellular Component: cytoplasm; extracellular space
Molecular Function: protein binding; serine-type endopeptidase inhibitor activity
Biological Process: cell motility; extracellular matrix organization and biogenesis; morphogenesis of an epithelium; regulation of epithelial cell proliferation
Research Articles on MASPIN
Similar Products
Product Notes
The MASPIN serpinb5 (Catalog #AAA178287) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-MASPIN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MASPIN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Western Blot Concentration: 0.1-0.5ug/ml Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml Immunocytochemistry Concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the MASPIN serpinb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MASPIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
