Rabbit anti-Human MASP2 Polyclonal Antibody | anti-MASP2 antibody

Anti-MASP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102058

• Gene Names: MASP2; sMAP; MAP19; MASP-2; MASP1P1
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MASP2; Polyclonal Antibody; Anti-MASP2 Antibody; Rabbit Polyclonal; MASP2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-MAP19 Antibody; Anti-MASP-2 Antibody; Anti-MASP1P1 Antibody; Anti-sMAP Antibody; anti-MASP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MASP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MASP2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human MASP2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MASP2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MASP2 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain Tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:76 kDaObserved band size:76 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MASP2 antibody

MASP-2 is the serine protease responsible for lectin pathway activation.MASP-2 is a key protein of the lectin pathway of complement system. Several MASP2 polymorphisms were associated with MASP-2 serum levels or functional activity. The gene MASP2 (mannan-binding lectin (MBL)-associated serine protease 2) encodes two proteins, MASP-2 and MAp19 (MBL-associated protein of 19 kDa), bound in plasma to MBL and ficolins.

NCBI and Uniprot Product Information

• NCBI GI #: 21264363
• NCBI GeneID: 10747
• NCBI Accession #: NP_006601.2
• NCBI GenBank Nucleotide #: NM_006610.3
• UniProt Accession #: O00187; O75754; Q5TEQ5; Q5TER0; Q96QG4; Q9BZH0; Q9H498; Q9H499; Q9UBP3; Q9UC48; A8K458; A8MWJ2
• Molecular Weight: 75,702 Da
• NCBI Official Full Name: mannan-binding lectin serine protease 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: mannan-binding lectin serine peptidase 2
• NCBI Official Symbol: MASP2
• NCBI Official Synonym Symbols: sMAP; MAP19; MASP-2; MASP1P1
• NCBI Protein Information: mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; ma
• UniProt Protein Name: Mannan-binding lectin serine protease 2
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP2
• UniProt Synonym Gene Names: MASP-2
• UniProt Entry Name: MASP2_HUMAN

NCBI Description

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

Uniprot Description

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.104; Secreted

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding

Biological Process: innate immune response; proteolysis; complement activation, lectin pathway; complement activation, classical pathway; complement activation

Disease: Masp2 Deficiency