Western Blot (WB)
(Western blot analysis of anti-EMK Antibody (C-term) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse MARK2 Polyclonal Antibody | anti-MARK2 antibody
MARK2, CT (MAP/microtubule Affinity-regulating Kinase 2, ELKL Motif Kinase 1, EMK1, EMK-1, PAR1 Homolog, PAR1 Homolog b, Par1b, Par-1b, PAR-1, Serine/threonine-protein Kinase MARK2) (FITC)
Gene Names
MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MARK2; Polyclonal Antibody; CT (MAP/microtubule Affinity-regulating Kinase 2; ELKL Motif Kinase 1; EMK1; EMK-1; PAR1 Homolog; PAR1 Homolog b; Par1b; Par-1b; PAR-1; Serine/threonine-protein Kinase MARK2) (FITC); EC=2.7.11.1; EC=2.7.11.26; MGC99619; anti-MARK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EMK. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
788
Applicable Applications for anti-MARK2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:250-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human EMK (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-EMK Antibody (C-term) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-EMK Antibody (C-term) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(EMK Antibody (V581) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the EMK antibody detected the EMK protein (arrow).)
Western Blot (WB)
(EMK Antibody (V581) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the EMK antibody detected the EMK protein (arrow).)
Product Categories/Family for anti-MARK2 antibody
References
1. Blume-Jensen P, et al. Nature 2001. 411: 355. 2. Cantrell D, J. Cell Sci. 2001. 114: 1439. 3. Jhiang S Oncogene 2000. 19: 5590. 4. Manning G, et al. Science 2002. 298: 1912. 5. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. 6.Robertson, S. et al. Trends Genet. 2000. 16: 368. 7.Robinson D, et al. Oncogene 2000. 19: 5548. 8.Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. 9.Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. 10.Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
83.204
NCBI Official Full Name
Serine/threonine-protein kinase MARK2
NCBI Official Synonym Full Names
microtubule affinity regulating kinase 2
NCBI Official Symbol
MARK2
NCBI Official Synonym Symbols
EMK1; EMK-1; PAR-1; Par1b; Par-1b
NCBI Protein Information
serine/threonine-protein kinase MARK2
Protein Family
NCBI Description
This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Research Articles on MARK2
Similar Products
Product Notes
The MARK2 (Catalog #AAA6486850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARK2, CT (MAP/microtubule Affinity-regulating Kinase 2, ELKL Motif Kinase 1, EMK1, EMK-1, PAR1 Homolog, PAR1 Homolog b, Par1b, Par-1b, PAR-1, Serine/threonine-protein Kinase MARK2) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MARK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:250-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MARK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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