Rabbit anti-Human MARK Polyclonal Antibody | anti-MARK antibody

MARK, CT (MARK1, KIAA1477, MARK, Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c) (FITC)

Cat. Num. AAA6319026

• Gene Names: MARK1; MARK; Par1c; Par-1c
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MARK; Polyclonal Antibody; CT (MARK1; KIAA1477; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c) (FITC); MARK1; PAR1 homolog c; anti-MARK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MARK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MARK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MARK.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MARK Antibody (S658) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MARK antibody detected the MARK protein (arrow).)

Immunohistochemistry (IHC)

(MARK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MARK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MARK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-MARK antibody

Antibodies; Protein Kinases

References

Drewes, G., et al., Cell 89(2):297-308 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 153791472
• NCBI GeneID: 4139
• NCBI Accession #: NP_061120
• NCBI GenBank Nucleotide #: NM_018650
• UniProt Accession #: Q9P0L2; Q2HIY1; Q5VTF9; Q5VTG0; Q96SW9; Q9P251; D3DTB0; D3DTB1
• Molecular Weight: 84,911 Da
• NCBI Official Full Name: serine/threonine-protein kinase MARK1 isoform 2
• NCBI Official Synonym Full Names: MAP/microtubule affinity-regulating kinase 1
• NCBI Official Symbol: MARK1
• NCBI Official Synonym Symbols: MARK; Par1c; Par-1c
• NCBI Protein Information: serine/threonine-protein kinase MARK1; PAR1 homolog c
• UniProt Protein Name: Serine/threonine-protein kinase MARK1
• UniProt Gene Name: MARK1
• UniProt Synonym Gene Names: Par-1c; Par1c
• UniProt Entry Name: MARK1_HUMAN

Uniprot Description

MARK1: a member of the CAMKL family of protein kinases. The MARK group of kinases is apparently involved in the control of cell polarity, microtubule stability and cancer. MARK1 forms a tight association with Tau in neurofibrillary tangles in Alzheimer disease tissue.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; tau-protein kinase activity; magnesium ion binding; ATP binding

Biological Process: Wnt receptor signaling pathway; neuron migration; cytoskeleton organization and biogenesis; protein amino acid phosphorylation