Rabbit anti-Human MARCKSL1 Polyclonal Antibody | anti-MARCKSL1 antibody

MARCKSL1, CT (MARCKSL1, MLP, MRP, MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate) (FITC)

Cat. Num. AAA6319004

• Gene Names: MARCKSL1; F52; MLP; MRP; MLP1; MACMARCKS
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MARCKSL1; Polyclonal Antibody; CT (MARCKSL1; MLP; MRP; MARCKS-related protein; MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate) (FITC); Macrophage myristoylated alanine-rich C kinase substrate; anti-MARCKSL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MARCKSL1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MARCKSL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human MARCKSL1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MARCKSL1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MARCKSL1 antibody detected the MARCKSL1 protein (arrow).)

Related Product Information for anti-MARCKSL1 antibody

MARCKSL1 may be involved in coupling the protein kinase C and calmodulin signal transduction systems.

Product Categories/Family for anti-MARCKSL1 antibody

Antibodies; Protein Kinases

References

Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Jordanova, A., et al. Nat. Genet. 38(2):197-202(2006). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 13491174
• NCBI GeneID: 65108
• NCBI Accession #: NP_075385.1
• NCBI GenBank Nucleotide #: NM_023009.6
• UniProt Accession #: P49006; Q5TEE6; Q6NXS5; D3DPQ0
• Molecular Weight: 19,529 Da
• NCBI Official Full Name: MARCKS-related protein
• NCBI Official Synonym Full Names: MARCKS-like 1
• NCBI Official Symbol: MARCKSL1
• NCBI Official Synonym Symbols: F52; MLP; MRP; MLP1; MACMARCKS
• NCBI Protein Information: MARCKS-related protein; MARCKS-like protein 1; mac-MARCKS; macrophage myristoylated alanine-rich C kinase substrate
• UniProt Protein Name: MARCKS-related protein
• Protein Family: MARCKS-related protein
• UniProt Gene Name: MARCKSL1
• UniProt Synonym Gene Names: MLP; MRP; Mac-MARCKS; MacMARCKS
• UniProt Entry Name: MRP_HUMAN

NCBI Description

This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012]

Uniprot Description

MLP: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. Belongs to the MARCKS family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calmodulin binding; actin binding

Biological Process: positive regulation of cell proliferation