Rabbit MARCH8 Polyclonal Antibody | anti-MARCH8 antibody

MARCH8 Antibody

Cat. Num. AAA9414421

• Gene Names: MARCH8; MIR; CMIR; c-MIR; RNF178; MARCH-VIII
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: MARCH8; Polyclonal Antibody; MARCH8 Antibody; Membrane-associated ring finger 8; E3 ubiquitin-protein ligase MARCH8; RING finger protein 178; c-MIR; Cellular modulator of immune recognition; anti-MARCH8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 291

• Applicable Applications for anti-MARCH8 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide from near the carboxy terminus of human MARCH8.
• Target Name: MARCH8
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 ug/ml and (B) 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 ug/mL.)

Related Product Information for anti-MARCH8 antibody

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Product Categories/Family for anti-MARCH8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 50539410
• NCBI GeneID: 220972
• NCBI Accession #: NP_659458
• NCBI GenBank Nucleotide #: NM_145021.5
• Molecular Weight: 63,319 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH8 isoform a
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
• NCBI Official Symbol: MARCH8
• NCBI Official Synonym Symbols: MIR; CMIR; c-MIR; RNF178; MARCH-VIII
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH8
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH8
• UniProt Gene Name: MARCH8
• UniProt Synonym Gene Names: MIR; RNF178; c-MIR; MARCH-VIII
• UniProt Entry Name: MARH8_HUMAN

NCBI Description

MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

Uniprot Description

MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.

Protein type: Membrane protein, integral; Ubiquitin conjugating system; Membrane protein, multi-pass; Ligase; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: cytoplasmic vesicle membrane; early endosome membrane; lysosome; lysosomal membrane; integral to membrane; endosome

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; MHC class II protein binding; ligase activity

Biological Process: protein polyubiquitination; antigen processing and presentation of peptide antigen via MHC class II; negative regulation of MHC class II biosynthetic process