Rabbit anti-Mouse MARCH1 Polyclonal Antibody | anti-MARCH1 antibody

MARCH1, ID (MARCH1, RNF171, E3 ubiquitin-protein ligase MARCH1, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, RING finger protein 171) (PE)

Cat. Num. AAA6318934

• Gene Names: MARCH1; RNF171; MARCH-I
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MARCH1; Polyclonal Antibody; ID (MARCH1; RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171) (PE); RING finger protein 171; anti-MARCH1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MARCH1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human MARCH1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).)

Related Product Information for anti-MARCH1 antibody

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).

Product Categories/Family for anti-MARCH1 antibody

Antibodies; Ubiquitin Ligases

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Thibodeau, J., et al. Eur. J. Immunol. 38(5):1225-1230(2008). De Gassart, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3491-3496(2008). Bartee, E., et al. J. Virol. 78(3):1109-1120(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 8923613
• NCBI GeneID: 55016
• NCBI Accession #: NP_060393.1; NP_001159845.1
• NCBI GenBank Nucleotide #: NM_017923.3
• UniProt Accession #: Q8TCQ1; Q9NWR0; D3DP29
• Molecular Weight: 30,873 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH1 isoform 2
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 1
• NCBI Official Symbol: MARCH1
• NCBI Official Synonym Symbols: RNF171; MARCH-I
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH1
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH1
• UniProt Gene Name: MARCH1
• UniProt Synonym Gene Names: RNF171; MARCH-I
• UniProt Entry Name: MARH1_HUMAN

NCBI Description

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

Uniprot Description

MARCH1: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligase; Membrane protein, multi-pass; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q32.2

Cellular Component: endoplasmic reticulum membrane; cytoplasmic vesicle membrane; lysosomal membrane; lysosome; late endosome membrane; early endosome membrane; integral to membrane; plasma membrane; trans-Golgi network membrane; endosome

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; MHC protein binding; ligase activity

Biological Process: protein polyubiquitination; antigen processing and presentation of peptide antigen via MHC class II; immune response