Western Blot (WB)
(MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).)
Rabbit anti-Mouse 3/1/2013 Polyclonal Antibody
MARCH1, ID (MARCH1, RNF171, E3 ubiquitin-protein ligase MARCH1, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, RING finger protein 171)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
3/1/2013; Polyclonal Antibody; MARCH1; ID (MARCH1; RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171); Anti -MARCH1; anti-3/1/2013 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-3/1/2013 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human MARCH1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).)
Western Blot (WB)
(MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).)
Related Product Information for anti-3/1/2013 antibody
MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).
Product Categories/Family for anti-3/1/2013 antibody
Similar Products
Product Notes
The 3/1/2013 (Catalog #AAA6011819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH1, ID (MARCH1, RNF171, E3 ubiquitin-protein ligase MARCH1, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, RING finger protein 171) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 3/1/2013 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the 3/1/2013 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "3/1/2013, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
