Immunohistochemistry (IHC)
(Anti-MAPKAPK5/PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Goat MAPKAPK5/PRAK Polyclonal Antibody | anti-MAPKAPK5/PRAK antibody
Anti-MAPKAPK5/PRAK Antibody (aa306-318)
Predicted: Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Monkey
Predicted: Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Monkey
IHC-P: 5 ug/ml
WB: 0.5-1 ug/ml
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-MAPKAPK5/PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-MAPKAPK5/PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with EB11928 (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.)
Western Blot (WB)
(MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with EB11928 (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.)
Western Blot (WB)
(MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5/PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).)
Western Blot (WB)
(MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5/PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]
Uniprot Description
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Research Articles on MAPKAPK5/PRAK
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Product Notes
The MAPKAPK5/PRAK mapkapk5 (Catalog #AAA2400885) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAPKAPK5/PRAK Antibody (aa306-318) reacts with Human Predicted: Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MAPKAPK5/PRAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:32000 IHC-P: 5 ug/ml WB: 0.5-1 ug/ml. Researchers should empirically determine the suitability of the MAPKAPK5/PRAK mapkapk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPKAPK5/PRAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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