Western Blot (WB)
(Western blot analysis of C6 cell lysate untreated or treated with anisomycin using Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody)
Rabbit MAPK8/MAPK9/MAPK10 Polyclonal Antibody | anti-MAPK8 antibody
Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223)
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of C6 cell lysate untreated or treated with anisomycin using Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody)
Western Blot (WB)
(Western blot analysis of C6 cell lysate untreated or treated with anisomycin using Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody)
As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).
Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012).
The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
Uniprot Description
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Research Articles on MAPK8
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Product Notes
The MAPK8 mapk9 (Catalog #AAA225326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) reacts with Rat, Human (Expected from Sequence), Mouse (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK8/MAPK9/MAPK10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MAPK8 mapk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK8/MAPK9/MAPK10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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