Rabbit MAPK7 Polyclonal Antibody | anti-MAPK7 antibody

Anti-MAPK7 Antibody

Cat. Num. AAA419989

• Gene Names: Mapk7; BMK1; ERK5; BMK-1; ERK-5; PRKM7; Erk5-T; b2b2346Clo
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: MAPK7; Polyclonal Antibody; Anti-MAPK7 Antibody; anti-MAPK7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using MAPK7 antibody. Immunohistochemistry of paraffin-embedded human esophageal cancer using MAPK7 antibody at dilution of 1:100 (400x lens). Immunohistochemistry of paraffin-embedded human esophagus using MAPK7 antibody at dilution of 1:100 (400x lens).
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 703

• Applicable Applications for anti-MAPK7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant protein of human MAPK7
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-MAPK7 antibody

ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1,5-7). Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism (2-4). For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (2, 3). ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation (4). In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival (11, 12). ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function (8-10). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis (1,5).

NCBI and Uniprot Product Information

• NCBI GI #: 597518001
• NCBI GeneID: 23939
• NCBI Accession #: NP_001277966.1
• NCBI GenBank Nucleotide #: NM_001291037.1
• UniProt Accession #: Q9WVS8; Q3U2N7; Q3UG52; Q497T0; Q5NCN6; Q5NCN7; Q5NCN9; Q6QLU8; Q9R1D9; Q9WVF3; Q9WVF4
• Molecular Weight: 82,314 Da
• NCBI Official Full Name: mitogen-activated protein kinase 7 isoform d
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 7
• NCBI Official Symbol: Mapk7
• NCBI Official Synonym Symbols: BMK1; ERK5; BMK-1; ERK-5; PRKM7; Erk5-T; b2b2346Clo
• NCBI Protein Information: mitogen-activated protein kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase 7
• UniProt Gene Name: Mapk7
• UniProt Synonym Gene Names: Bmk1; Erk5; MAP kinase 7; MAPK 7; BMK-1; ERK-5

Uniprot Description

ERK5: a protein kinase of the MAPK family. Phosphorylated and activated by MEK5. Involved in the signaling processes downstream of various receptors including receptor tyrosine kinases and G protein-coupled receptors. Translocates to cell nucleus in response to extracelluar signals, where it regulates gene expression by phosphorylating various transcription factors. Mouse knockout shows a role in development and integrity of blood vessels. May block apoptosis in endothelial cells. Required for transduction of EGF growth signal, constitutively activated in ErbB2-overexpressing breast cancer, and downstream of activated Mek5 in metastatic prostate cancerFour alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11|11 B2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; PML body

Molecular Function: ATP binding; MAP kinase activity; mitogen-activated protein kinase binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: negative regulation of apoptosis; negative regulation of heterotypic cell-cell adhesion; negative regulation of MAP kinase activity; negative regulation of NFAT protein import into nucleus; peptidyl-serine phosphorylation; positive regulation of protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of angiogenesis