MAPK7 Antibody | anti-MAPK7 antibody

MAPK7 Antibody

Cat. Num. AAA8516305

• Gene Names: MAPK7; BMK1; ERK4; ERK5; PRKM7
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: MAPK7; MAPK7 Antibody; BMK1; ERK4; ERK5; PRKM7; anti-MAPK7 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: MAPK7 Antibody detects endogenous levels of total MAPK7
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 816

• Applicable Applications for anti-MAPK7 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human MAPK7
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts ofvarious celllines, using MAPK7 antibody.)

Related Product Information for anti-MAPK7 antibody

ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1, 5-7). Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism (2-4). For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (2, 3). ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation (4). In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival (11, 12). ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function (8-10). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis (1, 5).

NCBI and Uniprot Product Information

• NCBI GI #: 20986497
• NCBI GeneID: 5598
• NCBI Accession #: NP_002740.2
• NCBI GenBank Nucleotide #: NM_002749.3
• UniProt Accession #: Q13164; Q16634; Q59F50; Q6QLU7; Q7L4P4; Q969G1; Q96G51
• Molecular Weight: 89kDa
• NCBI Official Full Name: mitogen-activated protein kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 7
• NCBI Official Symbol: MAPK7
• NCBI Official Synonym Symbols: BMK1; ERK4; ERK5; PRKM7
• NCBI Protein Information: mitogen-activated protein kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase 7
• UniProt Gene Name: MAPK7
• UniProt Synonym Gene Names: BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.