Rabbit MAPK3 / ERK1 Polyclonal Antibody | anti-MAPK3 antibody

Rabbit Polyclonal to Human MAPK3 / ERK1

Cat. Num. AAA241505

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: MAPK3 / ERK1; Polyclonal Antibody; Rabbit Polyclonal to Human MAPK3 / ERK1; Anti-MAPK3 / ERK1 Antibody (Internal) IHC-plus; MAPK3; ERK-1; ERT2; HUMKER1A; HS44KDAP; MAP kinase 1; MAP kinase isoform p44; MAPK 1; MAPK 3; p44; p44MAPK; p44-MAPK; p44ERK1; Insulin-stimulated MAP2 kinase; MAP kinase 3; p44-ERK1; ERK1; PRKM3; Human MAPK3; anti-MAPK3 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MAPK3 / ERK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (70%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 357

• Applicable Applications for anti-MAPK3 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (4 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Bovine, Hamster, Elephant, Panda, Horse, Pig (95%).
• Immunogen Type: Synthetic peptide
• Immunogen: MAPK3 / ERK1 antibody was raised against synthetic 20 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Bovine, Hamster, Elephant, Panda, Horse, Pig (95%); Dog, Rabbit, Opossum, Platypus (90%); Bat (85%).
• Antigen Modification: Internal
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-MAPK3 / ERK1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-MAPK3 / ERK1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-MAPK3 / ERK1 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-MAPK3 / ERK1 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-MAPK3 / ERK1 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-MAPK3 antibody

ERK1, a MAPK -type protein kinase, is a member of the Ras-MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins, and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK1 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK1 causing dissociation of the MEK/ERK complex.

Product Categories/Family for anti-MAPK3 antibody

Family: Protein Kinase; Subfamily: MAPK

NCBI and Uniprot Product Information

• NCBI GI #: 91718897
• NCBI GeneID: 5595
• NCBI Accession #: NP_001035145.1
• NCBI GenBank Nucleotide #: NM_001040056.2
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• Molecular Weight: 40,088 Da
• NCBI Official Full Name: mitogen-activated protein kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: Golgi apparatus; focal adhesion; mitochondrion; early endosome; nuclear envelope; pseudopodium; caveola; cytosol; microtubule cytoskeleton; nucleoplasm; cytoskeleton; late endosome; nucleus

Molecular Function: MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding

Biological Process: axon guidance; activation of MAPKK activity; DNA damage induced protein phosphorylation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of histone phosphorylation; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; gene expression; toll-like receptor 9 signaling pathway; positive regulation of protein amino acid phosphorylation; transcription initiation from RNA polymerase I promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; phosphorylation; regulation of cytoskeleton organization and biogenesis