Rabbit anti-Human MAP4K4 Polyclonal Antibody | anti-MAP4K4 antibody

MAP4K4, Phosphorylated (Ser629) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4, HPK/GCK-like Kinase HGK, HGK, KIAA0687, MAPK/ERK Kinase Kinase Kinase 4, MEK Kinase Kinase 4, MEKKK 4, MEKKK4, Nck-interacting Kinase, NIK) (Azide free) (HRP)

Cat. Num. AAA6488261

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MAP4K4; Polyclonal Antibody; Phosphorylated (Ser629) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4; HPK/GCK-like Kinase HGK; HGK; KIAA0687; MAPK/ERK Kinase Kinase Kinase 4; MEK Kinase Kinase 4; MEKKK 4; MEKKK4; Nck-interacting Kinase; NIK) (Azide free) (HRP); EC=2.7.11.1; FLH21957; FLJ10410; FLJ20373; FLJ90111; anti-MAP4K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP4K4 when phosphorylated at Ser629.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MAP4K4 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser629 of human MAP4K4 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-MAP4K4-pS629 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-MAP4K4 antibody

Antibodies; Protein Kinases

References

1. Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003). 2. Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999). 3. Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 336020358
• NCBI GeneID: 9448
• NCBI Accession #: NP_001229488.1
• NCBI GenBank Nucleotide #: NM_001242559.1
• UniProt Accession #: O95819; O75172; Q9NST7
• Molecular Weight: 142,101 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; hepatocyte progenitor kinase-like/germinal center kinase-like k
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; regulation of catalytic activity; regulation of JNK cascade; protein amino acid phosphorylation