Testing Data
(LPS stimulated rat splenocytes stained with Mouse anti Rat CD252:FITC (MBS224447))
Mouse CD252 Monoclonal Antibody | anti-CD252 antibody
MOUSE ANTI RAT CD252
Gene Names
Tnfsf4; Ox40l
Applications
Flow Cytometry, Functional Assay
Synonyms
CD252; Monoclonal Antibody; MOUSE ANTI RAT CD252; anti-CD252 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ATM-2
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
199
Applicable Applications for anti-CD252 antibody
Flow cytometry (FC/FACS)*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
ACI-S1 rat T-cell lymphoma cell line.
Buffer Solution
Buffer Solution
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(LPS stimulated rat splenocytes stained with Mouse anti Rat CD252:FITC (MBS224447))
Testing Data
(LPS stimulated rat splenocytes stained with Mouse anti Rat CD252:FITC (MBS224447))
Related Product Information for anti-CD252 antibody
Mouse anti Rat CD252 antibody, clone ATM-2 specifically recognizes rat CD252, otherwise known as OX40L or CD134L, a 22kDa type II transmembrane glycoprotein and member of the TNF superfamily, expressed by antigen-presenting cells including activated dendritic cells and B cells, and also by vascular endothelium. The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease. Mouse anti Rat CD252 antibody, clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,190 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 4
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 4
NCBI Official Symbol
Tnfsf4
NCBI Official Synonym Symbols
Ox40l
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4; OX40 ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 4
UniProt Gene Name
Tnfsf4
UniProt Synonym Gene Names
Ox40l; Txgp1; OX40L
UniProt Entry Name
TNFL4_RAT
NCBI Description
ligand for the tumor necrosis factor receptor OX40 (CD134); exhibits costimulatory activity for T-cell proliferation [RGD, Feb 2006]
Uniprot Description
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production ().
Research Articles on CD252
Similar Products
Product Notes
The CD252 tnfsf4 (Catalog #AAA219578) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD252 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD252 tnfsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD252, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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