Rabbit MAP3K9 Polyclonal Antibody | anti-MAP3K9 antibody

MAP3K9, CT (Mitogen-activated Protein Kinase Kinase Kinase 9, MEKK9, Mixed Lineage Kinase 1, MLK1, PRKE1) (PE)

Cat. Num. AAA6486505

• Gene Names: MAP3K9; MLK1; MEKK9; PRKE1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAP3K9; Polyclonal Antibody; CT (Mitogen-activated Protein Kinase Kinase Kinase 9; MEKK9; Mixed Lineage Kinase 1; MLK1; PRKE1) (PE); E130314H24Rik; EC=2.7.11.25; RGD1562149; anti-MAP3K9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLK1. Species Crossreactivity: mouse, rat.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1118

• Applicable Applications for anti-MAP3K9 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MLK1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-MLK1 Pab is used in Western blot to detect MLK1 in A375 cell lysate (lane 1) and rat testis tissue lysate (lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAP3K9 antibody

Antibodies; Protein Kinases

References

1. Durkin, J.T., et al., Biochemistry 43(51):16348-16355 (2004). 2. Gallo, K.A., et al., J. Biol. Chem. 269(21):15092-15100 (1994). 3. Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 52421790
• NCBI GeneID: 4293
• NCBI Accession #: NP_149132
• NCBI GenBank Nucleotide #: NP_149132.2
• UniProt Accession #: P80192
• Molecular Weight: 118.463
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 9 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 9
• NCBI Official Symbol: MAP3K9
• NCBI Official Synonym Symbols: MLK1; MEKK9; PRKE1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 9
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 9
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K9
• UniProt Synonym Gene Names: MLK1; PRKE1
• UniProt Entry Name: M3K9_HUMAN

Uniprot Description

MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 14q24.2

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; JUN kinase kinase kinase activity; protein-tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity