Rabbit anti-Human, Rat Dual specificity mitogen-activated protein kinase kinase 5 Polyclonal Antibody | anti-MAP2K5 antibody

Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 5 polyclonal Antibody(MAP2K5)

Cat. Num. AAA1493796

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Dual specificity mitogen-activated protein kinase kinase 5; Polyclonal Antibody; Rabbit anti-human Dual specificity mitogen-activated protein kinase kinase 5 polyclonal Antibody(MAP2K5); MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MAP2K5; MEK5; MKK5; PRKMK5; anti-MAP2K5 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 412

• Applicable Applications for anti-MAP2K5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-5000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Dual specificity mitogen-activated protein kinase kinase 5 protein(1-190aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9320 / sc-1287 / sc-10795
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Dual specificity mitogen-activated protein kinase kinase 5 antibody at 2ug/ml+rat heart tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 51,49,50,47 kDaObserved band size: 50 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testicle using MBS1493796 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gallbladder using MBS1493796 at dilution 1:100)

Related Product Information for anti-MAP2K5 antibody

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 332164798
• NCBI GeneID: 5607
• NCBI Accession #: NP_001193733.1
• NCBI GenBank Nucleotide #: NM_001206804.1
• UniProt Accession #: Q13163; Q92961; Q92962; B4DE43
• Molecular Weight: 46,131 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform C
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Uniprot Description

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Protein kinase, STE; STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: cytoplasm; nucleus; spindle

Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity

Biological Process: activation of MAPK activity; activation of protein kinase activity; heart development; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of mitotic cell cycle; signal transduction; stress-activated protein kinase signaling pathway