Rabbit anti-Human MAP2K2 Polyclonal Antibody | anti-MAP2K2 antibody

MAP2K2 (Dual Specificity Mitogen-activated Protein Kinase Kinase 2, MAP Kinase Kinase 2, MAPKK 2, ERK Activator Kinase 2, MAPK/ERK Kinase 2, MEK 2, FLJ26075, MEK2, MKK2, PRKMK2) APC

Cat. Num. AAA6384786

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAP2K2; Polyclonal Antibody; MAP2K2 (Dual Specificity Mitogen-activated Protein Kinase Kinase 2; MAP Kinase Kinase 2; MAPKK 2; ERK Activator Kinase 2; MAPK/ERK Kinase 2; MEK 2; FLJ26075; MEK2; MKK2; PRKMK2) APC; anti-MAP2K2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAP2K2 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length human MAP2K2, aa1-400 (NP_109587.1).
• Immunogen Sequence: MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MAP2K2 rabbit polyclonal antibody. Western Blot analysis of MAP2K2 expression in human kidney)

Western Blot (WB)

(Western Blot analysis of MAP2K2 expression in transfected 293T cell line by MAP2K2 polyclonal antibody. Lane 1: MAP2K2 transfected lysate (44.4kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified rabbit antibody to MAP2K2 on HeLa cell. [antibody concentration 10ug/ml])

Product Categories/Family for anti-MAP2K2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662
• UniProt Accession #: P36507
• Molecular Weight: 400
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
• UniProt Entry Name: MP2K2_HUMAN

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; peroxisomal membrane; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; extracellular region; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; ATP binding; PDZ domain binding

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of stress-activated MAPK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway

Disease: Cardiofaciocutaneous Syndrome 4