Mouse anti-Human, Mouse CD3e Monoclonal Antibody | anti-CD3e antibody

CD3e (PE)

Cat. Num. AAA6244546

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CD3e; Monoclonal Antibody; CD3e (PE); anti-CD3e antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5H55
• Specificity: Recognizes human CD3e, Mr 23kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CD3e antibody: Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Purified human CD3e (NP_000724)
• Conjugate: PE
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2254-61 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Arrow indicates CD3e (~23kD).)

Testing Data

(T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue with CMAC dye) activated by Staphylococcal enterotoxin E in contact with a single T-cell (a Jurkat cell). Panel B is an image of the two cells, stained with an anti-CD3ζ antiserum (a 1:3,000 dilution) and C2254-61. Visualization of CD3ζ or C2254-61 was with an anti-rabbit Alexa 488 (green) or anti-mouse Alexa 594 (red) conjugate, respectively; the yellow color is produced by co-localization of CD3ζ and C2254-61 staining. Data generously provided by Dr. Balbino Alarcon, Centro de Biologia Molecular, CSIC, Madrid, Spain)

Product Categories/Family for anti-CD3e antibody

Antibodies; CD Markers

References

1. Alarcon, B. et al., EMBO J. 10: 903-912, 1991. 2. Borroto, A. et al., J. Biol. Chem. 273: 12807-12816, 1998. 3. Gil, D. et al., Cell 109: 901-912, 2002. 4. Alarcon, B. et al., Immunol. Rev. 191: 38-46, 2003. 5. Werlen, G. et al., Science 299: 1859-1863a, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18