Rabbit anti-Human, Mouse MAP1LC3B Polyclonal Antibody | anti-MAP1LC3B antibody

MAP1LC3B (LC3B, Autophagy Related Protein LC3B, Autophagy Related Ubiquitin-like Modifier LC3B, MAP1 Light Chain 3-like Protein 2, Microtubule Associated Proteins 1A/1B Light Chain 3B, MAP1A/1B Light Chain 3B, MAP1A/MAP1B LC3B, Microtubule Associated Prot

Cat. Num. AAA613995

• Gene Names: MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Serum; Whole antisera
• Synonyms: MAP1LC3B; Polyclonal Antibody; MAP1LC3B (LC3B; Autophagy Related Protein LC3B; Autophagy Related Ubiquitin-like Modifier LC3B; MAP1 Light Chain 3-like Protein 2; Microtubule Associated Proteins 1A/1B Light Chain 3B; MAP1A/1B Light Chain 3B; MAP1A/MAP1B LC3B; Microtubule Associated Prot; Anti -MAP1LC3B (LC3B; anti-MAP1LC3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This antibody is specific for the B isoform of LC3. Species Crossreactivity: This antibody reacts with the human and mouse proteins. Other species have not been tested.
• Purity/Purification: Serum; Whole antisera

• Applicable Applications for anti-MAP1LC3B antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot Immunohistochemistry and Immunocytochemistry.
• Immunogen: A synthetic peptide made to the N-terminal region of the human LC3, isoform B protein.
• Cellular Localization: LC3 I: cytoplasmic, LC3 II: Intracytoplasmic membrane; lipid anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid anchor.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-MAP1LC3B antibody

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably

Product Categories/Family for anti-MAP1LC3B antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12383056
• NCBI GeneID: 81631
• NCBI Accession #: NP_073729.1
• NCBI GenBank Nucleotide #: NM_022818.4
• UniProt Accession #: Q9GZQ8; Q6NW02
• Molecular Weight: 14,688 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 beta
• NCBI Official Symbol: MAP1LC3B
• NCBI Official Synonym Symbols: LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_HUMAN

NCBI Description

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Uniprot Description

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Vesicle; Ubiquitin-like modifier; Microtubule-binding; Autophagy

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation