Rabbit anti-Human MAP1D Polyclonal Antibody | anti-METAP1D antibody

Anti-MAP1D Antibody, Rabbit Polyclonal

Cat. Num. AAA8104234

• Gene Names: METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: MAP1D; Polyclonal Antibody; Anti-MAP1D Antibody; Rabbit Polyclonal; MAP1D Antibody; Rabbit PAb; Antigen Affinity Purified; methionyl aminopeptidase type 1D (mitochondrial); Anti-MAP 1D Antibody; Anti-MetAP 1D Antibody; Anti-Metap1l Antibody; anti-METAP1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MAP1D
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-METAP1D antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Human MAP1D Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MAP1D (rh MAP1D). MAP1D specific IgG was purified by Human MAP1D affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-METAP1D antibody

Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independent growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese, or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.

References

Serero A., et al., (2003), An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway. J. Biol. Chem. 278:52953-52963.Leszczyniecka M., et al., (2006), MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer.Oncogene 25:3471-3478.Vaca Jacome A.S., et al., (2015), N-terminome analysis of the human mitochondrial proteome.Proteomics 15:2519-2524.

NCBI and Uniprot Product Information

• NCBI GI #: 40385867
• NCBI GeneID: 254042
• NCBI Accession #: NP_954697.1
• NCBI GenBank Nucleotide #: NM_199227.1
• UniProt Accession #: Q6UB28; Q1WNX3
• Molecular Weight: 37,088 Da
• NCBI Official Full Name: methionine aminopeptidase 1D, mitochondrial
• NCBI Official Synonym Full Names: methionyl aminopeptidase type 1D (mitochondrial)
• NCBI Official Symbol: METAP1D
• NCBI Official Synonym Symbols: MAP1D; MAP 1D; Metap1l; MetAP 1D
• NCBI Protein Information: methionine aminopeptidase 1D, mitochondrial; CDS of metAP-3 within PCR fragment; MAP 1D; metAP 1D; methionyl aminopeptidase type 1D, mitochondrial; peptidase M 1D
• UniProt Protein Name: Methionine aminopeptidase 1D, mitochondrial
• UniProt Gene Name: METAP1D
• UniProt Entry Name: MAP12_HUMAN

NCBI Description

The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAP1D: Removes the N-terminal methionine from nascent proteins. May play a role in colon tumorigenesis. Belongs to the peptidase M24A family.

Protein type: EC 3.4.11.18; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification