Rabbit anti-Human MAP Kinase Kinase 5 Polyclonal Antibody | anti-MKK5 antibody

MAP Kinase Kinase 5 (Ser149) (Dual Specificity Mitogen-activated Protein Kinase Kinase 5, MAP2K5, MAPKK 5, MAPKK5, MKK5, PRKMK5, HsT17454, MAPK/ERK Kinase 5, MEK 5, MEK5) (PE)

Cat. Num. AAA6486824

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MAP Kinase Kinase 5; Polyclonal Antibody; MAP Kinase Kinase 5 (Ser149) (Dual Specificity Mitogen-activated Protein Kinase Kinase 5; MAP2K5; MAPKK 5; MAPKK5; MKK5; PRKMK5; HsT17454; MAPK/ERK Kinase 5; MEK 5; MEK5) (PE); Mitogen-activated Protein Kinase Kinase 5; EC=2.7.12.2; anti-MKK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K5.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MKK5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S149 of human MAP2K5 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAP2K5 (arrow) using rabbit polyclonal MAP2K5 Antibody (S149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MKK5 antibody

Antibodies; Protein Kinases

References

1. Raviv, Z., J. Cell. Sci. 117(PT 9), 1773-1784 (2004). 2. Kato, Y., EMBO J. 16(23), 7054-7066 (1997). 3. English, J.M., J. Biol. Chem. 274(44), 31588-31592 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4506101
• NCBI GeneID: 5607
• NCBI Accession #: NP_002748
• NCBI GenBank Nucleotide #: NM_002757
• UniProt Accession #: Q13163; Q92961; Q92962; B4DE43
• Molecular Weight: 46,131 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform B
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MEK 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5

Uniprot Description

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.