Rabbit anti-Human MAP Kinase Interacting Serine/threonine Kinase 2 Polyclonal Antibody | anti-MKNK2 antibody

MAP Kinase Interacting Serine/threonine Kinase 2, NT (MAP Kinase Signal-integrating Kinase 2, MAPK Signal-integrating Kinase 2, MKNK2, MNK2, GPRK7)

Cat. Num. AAA624464

• Gene Names: MKNK2; MNK2; GPRK7
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAP Kinase Interacting Serine/threonine Kinase 2; Polyclonal Antibody; NT (MAP Kinase Signal-integrating Kinase 2; MAPK Signal-integrating Kinase 2; MKNK2; MNK2; GPRK7); Anti -MAP Kinase Interacting Serine/threonine Kinase 2; anti-MKNK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MKNK2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MKNK2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human MKNK2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MKNK2 Pab is used in Western blot to detect MKNK2 in mouse lung tissue lysate.)

Related Product Information for anti-MKNK2 antibody

MAP kinase-interacting kinase 1 (Mnk1) and Mnk2, members of the Ser/Thr protein kinase family, bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2. Erk and p38 phosphorylate MNK1 and Mnk2, which stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E). Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. This ubiquitiously expressed protein appears to regulate transcription by phosphorylating eIF-4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta. Mnk2 has also been shown to interact with the C-terminal regions of eIF-4G1 and eIF-4G2.

Product Categories/Family for anti-MKNK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49119657
• NCBI GeneID: 2872
• UniProt Accession #: Q9HBH9; Q6GPI3; Q9HBH8; Q9UHR0; Q9Y2N6
• Molecular Weight: 51,875 Da
• NCBI Official Full Name: MAP kinase interacting serine/threonine kinase 2
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 2
• NCBI Official Symbol: MKNK2
• NCBI Official Synonym Symbols: MNK2; GPRK7
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 2; MAPK signal-integrating kinase 2; G protein-coupled receptor kinase 7; MAP kinase signal-integrating kinase 2
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 2
• Protein Family: MAP kinase-interacting serine/threonine-protein kinase
• UniProt Gene Name: MKNK2
• UniProt Synonym Gene Names: GPRK7; MNK2; MAPK signal-integrating kinase 2; Mnk2
• UniProt Entry Name: MKNK2_HUMAN

NCBI Description

This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]

Uniprot Description

Mnk2: a ubiquituous CAM kinase of the MAPKKAPK family. Regulates translation by phosphorylating S209 of eIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. The phosphorylation of eIF4E S209 by the MNK1/2 kinases is required for eIF4E's oncogenic action. Phosphorylates Spry2 on S112 and S121, leading to the ubiquitination and degradation of Spry2. Five splice-variant isoforms have been described.

Protein type: Kinase, protein; Translation; Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; PML body; cytoplasm; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding

Biological Process: regulation of translation; cell surface receptor linked signal transduction; hemopoiesis; protein amino acid phosphorylation