Western Blot (WB)
((0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MAN2A1 Polyclonal Antibody | anti-MAN2A1 antibody
Goat anti-MAN2A1 Antibody
Western Blot: Approx.150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx.98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts.
Western Blot (WB)
((0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing Man2A1 (RC220186) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing Man2A1 (RC220186) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq, Dec 2013]
Uniprot Description
MAN2A1: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. Belongs to the glycosyl hydrolase 38 family.
Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 3.2.1.114; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q21.3
Cellular Component: cis-Golgi network; Golgi membrane; integral to membrane; membrane
Molecular Function: carbohydrate binding; hydrolase activity, hydrolyzing N-glycosyl compounds; mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity; zinc ion binding
Biological Process: alveolus development; in utero embryonic development; liver development; mannose metabolic process; mitochondrion organization and biogenesis; N-glycan processing; positive regulation of neurogenesis; protein deglycosylation; respiratory gaseous exchange; retina morphogenesis in camera-type eye; vacuole organization and biogenesis
Research Articles on MAN2A1
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Product Notes
The MAN2A1 man2a1 (Catalog #AAA420121) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MAN2A1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAN2A1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx.150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx.98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human dermal fibroblasts. Researchers should empirically determine the suitability of the MAN2A1 man2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KISSDIKSQN R. It is sometimes possible for the material contained within the vial of "MAN2A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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