anti-Human, Rat Mammalian Degenerin 1 Polyclonal Antibody
Anti - Mammalian Degenerin 1 (MDGE1, ASIC2, BNaC1)
Reactivity
Human, Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Mammalian Degenerin 1; Polyclonal Antibody; Anti - Mammalian Degenerin 1 (MDGE1; ASIC2; BNaC1); Mammalian Degenerin 1 (MDGE1; anti-Mammalian Degenerin 1 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Rat control peptide is 100% conserved in human MDEG1/BNaC1/BNA1. No significant sequence homology is detected with other ASICs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-Mammalian Degenerin 1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
Immunogen
A 20 AA Peptide sequence near the extracellular, N-terminus, of rat ASIC-b
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Mammalian Degenerin 1 antibody
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.
Product Categories/Family for anti-Mammalian Degenerin 1 antibody
References
• 1. Waldmann, R et al (1996) J Biol. Chem. 271, 10433-10436; Price MP et al (1996) J Biol. Chem. 271, 7879-7882.
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Product Notes
The Mammalian Degenerin 1 (Catalog #AAA614698) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Mammalian Degenerin 1 (MDGE1, ASIC2, BNaC1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mammalian Degenerin 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. Researchers should empirically determine the suitability of the Mammalian Degenerin 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mammalian Degenerin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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