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Western Blot (WB) (Western Blot: Sample: Human Serum.)

Rabbit anti-Human Maltase Glucoamylase, Intestinal (MGA) Polyclonal Antibody | anti-MGA antibody

Polyclonal Antibody to Maltase Glucoamylase, Intestinal (MGA)

Gene Names
MGAM; MG; MGA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Synonyms
Maltase Glucoamylase; Intestinal (MGA); Polyclonal Antibody; Polyclonal Antibody to Maltase Glucoamylase; anti-MGA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a Rabbit polyclonal antibody raised against MGA. It has been selected for its ability to recognize MGA in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Form/Format
Supplied as solution form in 0.01 PBS,pH 7.4, containing 0.05%, Proclin 300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LTYQVEIS RQPFSIKVTR RSNNRVLFDS SIGPLLFADQ FLQLSTRLPS TNVYGLGEHV HQQYRHDMNW KTWPIFNRDT TPNGNGTNLY GAQTFFLCLE DASGLSFGVF LMNSNAMEVV LQPAPAITYR TIGGILDFYV FLGNTPEQVV QEYLELIGRP ALPSYWALGF HLSRYEYGTL DN
Sequence Length
1857
Applicable Applications for anti-MGA antibody
WB, IHC, ICC, IP
Application Notes
Western blotting: 0.5 ug/mL; 1:200-800
Immunohistochemistry: 5-20 ug/mL; 1:20-80
Immunocytochemistry: 5-20 ug/mL; 1:20-80

Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Organism species
Homo sapiens (Human)
Traits
Liquid
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040137
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use
Aliquot and store at -20°C for 12 months.

Stability test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropiate storage condition.

Western Blot (WB)

(Western Blot: Sample: Human Serum.)

Western Blot (WB) (Western Blot: Sample: Human Serum.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MGA, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant MGA, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
209,852 Da
NCBI Official Full Name
maltase-glucoamylase, intestinal
NCBI Official Synonym Full Names
maltase-glucoamylase
NCBI Official Symbol
MGAM
NCBI Official Synonym Symbols
MG; MGA
NCBI Protein Information
maltase-glucoamylase, intestinal
UniProt Protein Name
Maltase-glucoamylase, intestinal
UniProt Gene Name
MGAM
UniProt Synonym Gene Names
MGA; MGAML

NCBI Description

This gene encodes maltase-glucoamylase, which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase, but the proteins are only 59% homologous. Both are members of glycosyl hydrolase family 31, which has a variety of substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

MGAM: May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing. Belongs to the glycosyl hydrolase 31 family.

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.20; EC 3.2.1.3; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: apical plasma membrane; integral component of membrane; plasma membrane

Molecular Function: alpha-1,4-glucosidase activity; amylase activity; carbohydrate binding; catalytic activity; glucan 1,4-alpha-glucosidase activity; maltase activity

Biological Process: maltose metabolic process; neutrophil degranulation; polysaccharide digestion; starch catabolic process

Research Articles on MGA

Similar Products

Product Notes

The MGA mgam (Catalog #AAA2003845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Maltase Glucoamylase, Intestinal (MGA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Maltase Glucoamylase, Intestinal (MGA) can be used in a range of immunoassay formats including, but not limited to, WB, IHC, ICC, IP. Western blotting: 0.5 ug/mL; 1:200-800 Immunohistochemistry: 5-20 ug/mL; 1:20-80 Immunocytochemistry: 5-20 ug/mL; 1:20-80 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MGA mgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- LTYQVEIS RQPFSIKVTR RSNNRVLFDS SIGPLLFADQ FLQLSTRLPS TNVYGLGEHV HQQYRHDMNW KTWPIFNRDT TPNGNGTNLY GAQTFFLCLE DASGLSFGVF LMNSNAMEVV LQPAPAITYR TIGGILDFYV FLGNTPEQVV QEYLELIGRP ALPSYWALGF HLSRYEYGTL DN. It is sometimes possible for the material contained within the vial of "Maltase Glucoamylase, Intestinal (MGA), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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