Rabbit anti-Human, Mouse MAK Polyclonal Antibody | anti-MAK antibody

MAK, CT (Male Germ Cell-associated Kinase, Serine/threonine-protein Kinase MAK, dJ417M14.2) (APC)

Cat. Num. AAA6486364

• Gene Names: MAK; RP62; dJ417M14.2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAK; Polyclonal Antibody; CT (Male Germ Cell-associated Kinase; Serine/threonine-protein Kinase MAK; dJ417M14.2) (APC); EC=2.7.11.22; anti-MAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAK (arrow) using MAK Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(MAK Antibody (T593) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the MAK antibody detected the MAK protein (arrow).)

Product Categories/Family for anti-MAK antibody

Antibodies; Protein Kinases

References

1. Xia, L., et al., J. Biol. Chem. 277(38):35422-35433 (2002). 2. Taketo, M., et al., Genomics 19(2):397-398 (1994). 3. Jinno, A., et al., Mol. Cell. Biol. 13(7):4146-4156 (1993). 4. Bladt, F., et al., Differentiation 53(2):115-122 (1993). 5. Koji, T., et al., Cell Biochem. Funct. 10(4):273-279 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 334724436
• NCBI GeneID: 4117
• NCBI Accession #: NP_001229314.1
• NCBI GenBank Nucleotide #: NM_001242385.1
• UniProt Accession #: P20794; Q547D0; Q9NUH7; F1T0K6; G1FL29
• Molecular Weight: 70,581 Da
• NCBI Official Full Name: serine/threonine-protein kinase MAK isoform 2
• NCBI Official Synonym Full Names: male germ cell-associated kinase
• NCBI Official Symbol: MAK
• NCBI Official Synonym Symbols: RP62; dJ417M14.2
• NCBI Protein Information: serine/threonine-protein kinase MAK; serine/threonine protein kinase MAK; male germ cell-associated kinase retinal-enriched isoform
• UniProt Protein Name: Serine/threonine-protein kinase MAK
• Protein Family: Makatoxin
• UniProt Gene Name: MAK
• UniProt Entry Name: MAK_HUMAN

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; Protein kinase, CMGC; CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: centrosome; photoreceptor outer segment; photoreceptor inner segment; cytoplasm; midbody; nucleus

Molecular Function: protein binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of cell cycle; multicellular organismal development; protein amino acid autophosphorylation; photoreceptor cell maintenance; spermatogenesis; cell differentiation; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 62