<
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb.)
Mouse anti-Human, Rat GRP78 BiP Monoclonal Antibody | anti-HSPA5 antibody
GRP78 BiP (6H7) Mouse mAb
Gene Names
HSPA5; BIP; MIF2; GRP78
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
GRP78 BiP; Monoclonal Antibody; GRP78 BiP (6H7) Mouse mAb; HSPA5; GRP78;MIF2; BIP; Heat shock 70kDa protein 5; anti-HSPA5 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6H7-4B1-9H4
Specificity
The GRP78/Bip mouse monoclonal antibody can recognizeendogenous GRP78/Bip proteins.
Purity/Purification
Affinity purified
Form/Format
PBS (pH7.4) containing with 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-HSPA5 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1;000-2;000
IHC: 1:100-200
(Optimal dilutions should be determined by the end user)
IHC: 1:100-200
(Optimal dilutions should be determined by the end user)
Immunogen
Synthetic peptide conjugated to KLH.
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Endoplasmic reticulum
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb.)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells using Bip/GRP78 (6H7) Mouse mAb(dilution 1:100).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells using Bip/GRP78 (6H7) Mouse mAb(dilution 1:100).)
WB (Western Blot)
(Western blot detection of Bip/GRP78 in Jurkat,MCF7,SKBR-3,Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb(1:2000 diluted). Predicted band size:78KDa. Observed band size:78KDa.)
WB (Western Blot)
(Western blot detection of Bip/GRP78 in Jurkat,MCF7,SKBR-3,Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb(1:2000 diluted). Predicted band size:78KDa. Observed band size:78KDa.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
WB (Western Blot)
(Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2,000.)
WB (Western Blot)
(Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2,000.)
Related Product Information for anti-HSPA5 antibody
Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,333 Da
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
Similar Products
Product Notes
The HSPA5 hspa5 (Catalog #AAA23991) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GRP78 BiP (6H7) Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRP78 BiP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1;000-2;000 IHC: 1:100-200 (Optimal dilutions should be determined by the end user). Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP78 BiP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
