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Western Blot (WB) (Western BlotPositive WB detected in:Mouse brain tissueAll lanes: MAK antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71,74,67 kDaObserved band size: 71 kDa)

Rabbit anti-Human, Mouse MAK Polyclonal Antibody | anti-MAK antibody

MAK Antibody

Gene Names
MAK; RP62
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
MAK; Polyclonal Antibody; MAK Antibody; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase; anti-MAK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
583
Applicable Applications for anti-MAK antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant human Serine/threonine-protein kinase MAK protein (200-440AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-100432 / sc-130165 / sc-135298
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse brain tissueAll lanes: MAK antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71,74,67 kDaObserved band size: 71 kDa)

Western Blot (WB) (Western BlotPositive WB detected in:Mouse brain tissueAll lanes: MAK antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71,74,67 kDaObserved band size: 71 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7048600 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7048600 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of A549 cells using MBS7048600 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of A549 cells using MBS7048600 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-MAK antibody
Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,345 Da
NCBI Official Full Name
serine/threonine-protein kinase MAK isoform 2
NCBI Official Synonym Full Names
male germ cell associated kinase
NCBI Official Symbol
MAK
NCBI Official Synonym Symbols
RP62
NCBI Protein Information
serine/threonine-protein kinase MAK
UniProt Protein Name
Serine/threonine-protein kinase MAK
Protein Family
UniProt Gene Name
MAK
UniProt Entry Name
MAK_HUMAN

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.

Protein type: CMGC group; EC 2.7.11.22; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); RCK family

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: centrosome; midbody; nucleus; photoreceptor inner segment; photoreceptor outer segment

Molecular Function: protein binding; transcription coactivator activity

Biological Process: photoreceptor cell maintenance; protein amino acid autophosphorylation; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 62

Research Articles on MAK

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Product Notes

The MAK mak (Catalog #AAA7048600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 1000-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the MAK mak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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