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Western Blot (WB)
(Western Blot: Sample: Recombinant MHCDRb1, Mouse.)
Rabbit anti-Mouse Major Histocompatibility Complex Class II DR Beta 1 Polyclonal Antibody | anti-MHCDRb1 antibody
Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1)
Gene Names
H2-Eb1; Eb; Ia4; H2Eb; Ia-4; H-2Eb
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Major Histocompatibility Complex Class II DR Beta 1; Polyclonal Antibody; Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1); anti-MHCDRb1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MHCDRb1. It has beenselected for its ability to recognize MHCDRb1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-MHCDRb1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
MHCDRb1 (Val27~Ser264)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant MHCDRb1 (Val27~Ser264) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063699
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant MHCDRb1, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MHCDRb1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,049 Da
NCBI Official Full Name
H-2 class II histocompatibility antigen, E-D beta chain
NCBI Official Synonym Full Names
histocompatibility 2, class II antigen E beta
NCBI Official Symbol
H2-Eb1
NCBI Official Synonym Symbols
Eb; Ia4; H2Eb; Ia-4; H-2Eb
NCBI Protein Information
H-2 class II histocompatibility antigen, I-E beta chain
UniProt Protein Name
H-2 class II histocompatibility antigen, E-D beta chain
Uniprot Description
H2-Eb1(D):
Cellular Component: integral component of membrane; late endosome membrane; lysosomal membrane; membrane; MHC class II protein complex
Molecular Function: protein binding
Biological Process: antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; immune response; immune system process
Research Articles on MHCDRb1
Similar Products
Product Notes
The MHCDRb1 (Catalog #AAA2028163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Major Histocompatibility Complex Class II DR Beta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MHCDRb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Major Histocompatibility Complex Class II DR Beta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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