Western Blot (WB)
(MAGEE2 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAGEE2 antibody detected the MAGEE2 protein (arrow).)
Rabbit anti-Human MAGEE2 Polyclonal Antibody | anti-MAGEE2 antibody
MAGEE2, CT (MAGEE2, HCA3, Melanoma-associated antigen E2, Hepatocellular carcinoma-associated protein 3, MAGE-E2 antigen) (Azide free) (HRP)
Gene Names
MAGEE2; HCA3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MAGEE2; Polyclonal Antibody; CT (MAGEE2; HCA3; Melanoma-associated antigen E2; Hepatocellular carcinoma-associated protein 3; MAGE-E2 antigen) (Azide free) (HRP); MAGE-E2 antigen; anti-MAGEE2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MAGEE2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MAGEE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-513 amino acids from the C-terminal region of human MAGEE2.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MAGEE2 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAGEE2 antibody detected the MAGEE2 protein (arrow).)
Western Blot (WB)
(MAGEE2 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAGEE2 antibody detected the MAGEE2 protein (arrow).)
Related Product Information for anti-MAGEE2 antibody
The specific function of this protein remains unknown.
Product Categories/Family for anti-MAGEE2 antibody
References
Ross, M.T., et al. Nature 434(7031):325-337(2005). Chomez, P., et al. Cancer Res. 61(14):5544-5551(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,378 Da
NCBI Official Full Name
melanoma-associated antigen E2
NCBI Official Synonym Full Names
melanoma antigen family E, 2
NCBI Official Symbol
MAGEE2
NCBI Official Synonym Symbols
HCA3
NCBI Protein Information
melanoma-associated antigen E2; MAGE-E2 antigen; hepatocellular carcinoma-associated protein 3; hepatocellular carcinoma-associated protein HCA3
UniProt Protein Name
Melanoma-associated antigen E2
Protein Family
UniProt Gene Name
MAGEE2
UniProt Synonym Gene Names
HCA3
UniProt Entry Name
MAGE2_HUMAN
NCBI Description
This gene encodes a member of the E subfamily of MAGE (melanoma antigen-encoding gene) gene family. The gene is intronless and the encoded protein has two of the MAGE domains which are characteristic of MAGE family proteins. [provided by RefSeq, Sep 2011]
Uniprot Description
MAGE-E2:
Chromosomal Location of Human Ortholog: Xq13.3
Similar Products
Product Notes
The MAGEE2 magee2 (Catalog #AAA6318070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGEE2, CT (MAGEE2, HCA3, Melanoma-associated antigen E2, Hepatocellular carcinoma-associated protein 3, MAGE-E2 antigen) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAGEE2 magee2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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