Rabbit anti-Human MAGED1 Polyclonal Antibody | anti-MAGED1 antibody

MAGED1, ID (Melanoma-associated Antigen D1, MAGE-D1 Antigen, Neurotrophin Receptor-interacting MAGE Homolog, MAGE Tumor Antigen CCF, NRAGE, PRO2292, PP2250)

Cat. Num. AAA628231

• Gene Names: MAGED1; NRAGE; DLXIN-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAGED1; Polyclonal Antibody; ID (Melanoma-associated Antigen D1; MAGE-D1 Antigen; Neurotrophin Receptor-interacting MAGE Homolog; MAGE Tumor Antigen CCF; NRAGE; PRO2292; PP2250); Anti -MAGED1; anti-MAGED1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGED1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MAGED1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the center region of human MAGED1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGED1 Ctr Antibody is used in Western blot to detect MAGED1 in A549 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-MAGED1 antibody

MAGED1 is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway.

Product Categories/Family for anti-MAGED1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 52632381
• NCBI GeneID: 9500
• NCBI Accession #: NP_001005333.1
• NCBI GenBank Nucleotide #: NM_001005333.1
• UniProt Accession #: Q8TEN0; Q9Y5V3
• Molecular Weight: 56,313 Da
• NCBI Official Full Name: melanoma-associated antigen D1 isoform a
• NCBI Official Synonym Full Names: melanoma antigen family D, 1
• NCBI Official Symbol: MAGED1
• NCBI Official Synonym Symbols: NRAGE; DLXIN-1
• NCBI Protein Information: melanoma-associated antigen D1; MAGE-D1 antigen; OTTHUMP00000023302; OTTHUMP00000023303; OTTHUMP00000023304; OTTHUMP00000023305; MAGE tumor antigen CCF; neurotrophin receptor-interacting MAGE homolog
• UniProt Protein Name: FLJ00163 protein
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: FLJ00163
• UniProt Entry Name: Q8TEN0_HUMAN

NCBI Description

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

NRAGE: a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although NRAGE shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. Binds p75 neurotrophin receptor (p75NTR) and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; plasma membrane; chromatin; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; positive regulation of MAP kinase activity; regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; circadian regulation of gene expression; regulation of circadian rhythm; negative regulation of transcription, DNA-dependent; negative regulation of epithelial cell proliferation