Rabbit MAGEC2 Polyclonal Antibody | anti-MAGEC2 antibody

MAGEC2 Polyclonal Antibody

Cat. Num. AAA3006255

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAGEC2; Polyclonal Antibody; MAGEC2 Polyclonal Antibody; Melanoma-associated antigen C2; Cancer/testis antigen 10; CT10; Hepatocellular carcinoma-associated antigen 587; MAGE-C2 antigen; MAGE-E1 antigen; MAGE-C2; HCA587; MAGEE1; anti-MAGEC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAGEC2 polyclonal antibody detects endogenous levels of MAGEC2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 373

• Applicable Applications for anti-MAGEC2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 237-282 of Human MAGEC2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAGEC2 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:NIH-3T3 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-MAGEC2 antibody

MAGE-C2/C3 (Melanoma-associated antigen C2, Hepatocellular carcinoma-associated antigen 587) is a 373 amino acid protein encoded by the human gene MAGEC2. Genes of the MAGE family direct the expression of tumor antigens that are recognized on human melanomas by autologous cytolytic T lymphocytes. Among tumor tissues, MAGE-C2/C3 is frequently expressed in seminomas, melanomas, and bladder transitional cell carcinomas. It is also expressed in a significant fraction of head and neck carcinomas, breast carcinomas, nonsmall-cell lung carcinomas, and sarcomas. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression. MAGE-C2/C3 is not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. It is strongly expressed in spermatogonia and primary spermatocytes, however, at later stages of maturation, expression gradually decreases to become undetectable in mature spermatids.

NCBI and Uniprot Product Information

• NCBI GI #: 7706605
• NCBI Accession #: NP_057333.1
• NCBI GenBank Nucleotide #: NP_057333.1
• UniProt Accession #: Q9UBF1
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: melanoma-associated antigen C2
• UniProt Protein Name: Melanoma-associated antigen C2
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEC2
• UniProt Synonym Gene Names: HCA587; MAGEE1; CT10
• UniProt Entry Name: MAGC2_HUMAN

Uniprot Description

MAGE-C2: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: cellular protein catabolic process; positive regulation of ubiquitin-protein ligase activity