Rabbit anti-Human MAGEA2 Polyclonal Antibody | anti-MAGEA2 antibody

MAGEA2, NT (Melanoma-associated Antigen 2, MAGE-2 Antigen, Cancer/Testis Antigen 1.2, CT1.2, MAGE2, MAGEA2A) (AP)

Cat. Num. AAA6486209

• Gene Names: MAGEA2; CT1.2; MAGE2; MAGEA2A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAGEA2; Polyclonal Antibody; NT (Melanoma-associated Antigen 2; MAGE-2 Antigen; Cancer/Testis Antigen 1.2; CT1.2; MAGE2; MAGEA2A) (AP); anti-MAGEA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGEA2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MAGEA2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MAGEA2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGEA2 N-term Antibody is used in Western blot to detect MAGEA2 in HL60 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAGEA2 antibody

Antibodies; Cancer Markers

References

1. Rogner, U.C., et al., Genomics 29(3):725-731 (1995). 2. De Smet, C., et al., Immunogenetics 39(2):121-129 (1994). 3. De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994). 4. van der Bruggen, P., et al., Science 254(5038):1643-1647 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4885465
• NCBI GeneID: 4101
• NCBI Accession #: NP_005352
• NCBI GenBank Nucleotide #: NM_005361
• UniProt Accession #: P43356
• Molecular Weight: 35kDa
• NCBI Official Full Name: melanoma-associated antigen 2
• NCBI Official Synonym Full Names: MAGE family member A2
• NCBI Official Symbol: MAGEA2
• NCBI Official Synonym Symbols: CT1.2; MAGE2; MAGEA2A
• NCBI Protein Information: melanoma-associated antigen 2
• UniProt Protein Name: Melanoma-associated antigen 2
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA2
• UniProt Synonym Gene Names: MAGE2; MAGEA2A; CT1.2
• UniProt Entry Name: MAGA2_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-A2: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T- lymphocytes.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: PML body; nucleus

Molecular Function: protein binding; histone deacetylase binding; ubiquitin protein ligase binding

Biological Process: transcription, DNA-dependent; cellular protein catabolic process; positive regulation of ubiquitin-protein ligase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein sumoylation