Rabbit anti-Human, Mouse MAFA Polyclonal Antibody | anti-MAFA antibody

Anti-MAFA Antibody

Cat. Num. AAA1753121

• Gene Names: MAFA; INSDM; hMafA; RIPE3b1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: MAFA; Polyclonal Antibody; Anti-MAFA Antibody; Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1 factor; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; anti-MAFA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 353

• Applicable Applications for anti-MAFA antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IFC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: Concentration: 0.25-0.5ug/ml; Tested Species: (Human); IHC-P (Embedded Section): Concentration: 0.5-1ug/ml; Tested Species: (Human, Mouse); IF/ICC: Concentration: 2ug/ml; Tested Species: Human; FC: Concentration: 1-3ug/1x10^6 cells; Tested Species: (Human); Direct ELISA: Concentration: 0.1-0.5ug/ml; Tested Species: (Human); ; Tested Species: In-house tested species with positive results.; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human MAFA recombinant protein (Position: A9-D308).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen store at -20°C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-MAFA antibody

Description: Rabbit IgG polyclonal antibody for MAFA detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse.

Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. It is mapped to 8q24.3.

References

1. Iacovazzo, D., Flanagan, S. E., Walker, E., Quezado, R., de Sousa Barros, F. A., Caswell, R., Johnson, M. B., Wakeling, M., Brandle, M., Guo, M., Dang, M. N., Gabrovska, P., and 14 others. MAFA missense mutation causes familial insulinomatosis and diabetes mellitus. Proc. Nat. Acad. Sci. 115: 1027-1032, 2018.
2. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002.
3. Tragl, K.-H., Mayr, W. R. Familial islet-cell adenomatosis. Lancet 318: 426-428, 1977. Note: Originally Volume II.

NCBI and Uniprot Product Information

• NCBI GI #: 71274111
• NCBI GeneID: 389692
• NCBI Accession #: NP_963883.2
• NCBI GenBank Nucleotide #: NP_963883.2
• UniProt Accession #: Q8NHW3
• NCBI Official Full Name: transcription factor MafA
• NCBI Official Synonym Full Names: MAF bZIP transcription factor A
• NCBI Official Symbol: MAFA
• NCBI Official Synonym Symbols: INSDM; hMafA; RIPE3b1
• NCBI Protein Information: transcription factor MafA
• UniProt Protein Name: Transcription factor MafA
• Protein Family: Transcription factor
• UniProt Gene Name: MAFA
• UniProt Entry Name: MAFA_HUMAN

NCBI Description

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: Transcription factor; DNA-binding; Tumor suppressor; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity

Biological Process: insulin secretion; nitric oxide mediated signal transduction; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; transcription from RNA polymerase II promoter