Western Blot (WB)
(Western Blot: Sample: Human U-87MG Cells.)
Rabbit anti-Human Cholinergic Receptor Polyclonal Antibody | anti-CHRNa4 antibody
Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human U-87MG Cells.)
Western Blot (WB)
(Western Blot: Sample: Human U-87MG Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CHRNa4, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CHRNa4, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Uniprot Description
nAChRA4: a ligand-gated ion channels that binds acetylcholine and mediates fast signal transmission at synapses. After binding acetylcholine, these pentameric receptors undergo an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. An integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor.
Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.
Chromosomal Location of Human Ortholog: 20q13.33
Cellular Component: acetylcholine-gated channel complex; cell junction; cell soma; dendrite; external side of plasma membrane; integral component of membrane; membrane; plasma membrane; postsynaptic membrane
Molecular Function: acetylcholine binding; acetylcholine receptor activity; acetylcholine-gated cation channel activity; ligand-gated ion channel activity; protein binding
Biological Process: B cell activation; behavioral response to nicotine; calcium ion transport; cognition; DNA repair; excitatory postsynaptic potential; inhibitory postsynaptic potential; ion transport; locomotory behavior; membrane depolarization; neurological system process; neuromuscular synaptic transmission; regulation of action potential; regulation of dopamine secretion; regulation of membrane potential; respiratory gaseous exchange; response to hypoxia; response to nicotine; response to oxidative stress; sensory perception of pain; signal transduction; synaptic transmission; synaptic transmission, cholinergic
Disease: Epilepsy, Nocturnal Frontal Lobe, 1; Tobacco Addiction, Susceptibility To
Research Articles on CHRNa4
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Product Notes
The CHRNa4 chrna4 (Catalog #AAA2032116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cholinergic Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CHRNa4 chrna4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholinergic Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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