Rabbit anti-Human Macrophage Inflammatory Protein 3 alpha Polyclonal Antibody | anti-CCL20 antibody

Macrophage Inflammatory Protein 3 alpha, Human (MIP-3a, Exodus, LARC)

Cat. Num. AAA624509

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Macrophage Inflammatory Protein 3 alpha; Polyclonal Antibody; Human (MIP-3a; Exodus; LARC); Anti -Macrophage Inflammatory Protein 3 alpha; anti-CCL20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Macrophage Inflammatory Protein-3 alpha (MIP-3a).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml.

• Applicable Applications for anti-CCL20 antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Neutralization, ELISA and Western Blot.; Dilution: Neutralization: 15-18ug/ml will yield one-half maximal inhibition (ND50) of the biological activity of hMIP-3-alpha (100ng/ml).; ELISA (Direct): 0.5ug/ml (100ul/well) allows the detection of 0.2-0.4ng/well of recombinant hMIP-3-alpha.; Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant hMIP-3-alpha is 1.5-3ng/lane, under either reducing or non-reducing conditions.; Optimal dilution determined by the researcher.
• Immunogen: Highly purified (> 98%), recombinant human Macrophage Inflammatory Protein-3 alpha (MIP-3a)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CCL20 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 1790925
• NCBI GeneID: 6364
• UniProt Accession #: P78556; Q53S51; Q99664
• Molecular Weight: 10,762 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20
• NCBI Protein Information: C-C motif chemokine 20; exodus-1; MIP-3-alpha; CC chemokine LARC; OTTHUMP00000164245; OTTHUMP00000204113; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; signal transduction; chemotaxis; positive regulation of interleukin-1 alpha biosynthetic process; inflammatory response; positive regulation of nitric-oxide synthase biosynthetic process