Rabbit anti-Mouse, Human LYVE1 Polyclonal Antibody | anti-LYVE1 antibody

Anti-LYVE1 Antibody (aa46-77) IHC-plus

Cat. Num. AAA249796

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: LYVE1; Polyclonal Antibody; Anti-LYVE1 Antibody (aa46-77) IHC-plus; Rabbit Polyclonal to Human LYVE1; Human LYVE1; CRSBP-1; CRSBP1; Hyaluronic acid receptor; LYVE-1; XLKD1; HAR; anti-LYVE1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Specificity: This XLKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-77 amino acids from the N-terminal region of human XLKD1.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Positions: 46-77

• Applicable Applications for anti-LYVE1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:100); WB (1:1000); Optimal dilution to be determined by the researcher.
• Immunogen: LYVE1 antibody was raised against kLH conjugated synthetic peptide selected from the N-terminal region of human XLKD1.
• Immunogen Description: KLH conjugated synthetic peptide selected from the N-terminal region of human XLKD1.
• Immunogen Type: Synthetic peptide
• Antigen Modification: aa46-77
• Target Species: Human
• Usage: This antibody does not work for IHC with samples fixed in 4% PFA.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Immunohistochemistry (IHC)

(Anti-LYVE1 / LYVE-1 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of XLKD1 (arrow) using rabbit polyclonal XLKD1 Antibody (C61). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.)

Western Blot (WB)

(XLKD1 Antibody western blot of Y79 cell line lysates (35 ug/lane). The XLKD1 antibody detected the XLKD1 protein (arrow).)

Western Blot (WB)

(The anti-XLKD1 N-term antibody is used in Western blot to detect XLKD1 in mouse liver tissue lysate.)

Related Product Information for anti-LYVE1 antibody

LYVE1 Antibody, CRSBP-1 Antibody, CRSBP1 Antibody, Hyaluronic acid receptor Antibody, LYVE-1 Antibody, XLKD1 Antibody, HAR Antibody Description: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

NCBI and Uniprot Product Information

• NCBI GI #: 40549451
• NCBI GeneID: 10894
• NCBI Accession #: NP_006682.2
• NCBI GenBank Nucleotide #: NM_006691.3
• Molecular Weight: 35,213 Da
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Lymphatic vessel endothelial hyaluronic acid receptor
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN

NCBI Description

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: integral to plasma membrane; membrane; plasma membrane

Molecular Function: hyaluronic acid binding; protein binding; receptor activity; transmembrane receptor activity

Biological Process: anatomical structure morphogenesis; carbohydrate metabolic process; cell motility; cell-matrix adhesion; glycosaminoglycan metabolic process; hyaluronan catabolic process; hyaluronan metabolic process; response to wounding; signal transduction; transport