Rabbit anti-Human LYPLA1 Polyclonal Antibody | anti-LYPLA1 antibody

LYPLA1, ID (LYPLA1, APT1, LPL1, Acyl-protein thioesterase 1, Lysophospholipase 1, Lysophospholipase I) (PE)

Cat. Num. AAA6317724

• Gene Names: LYPLA1; LPL1; APT-1; hAPT1; LYSOPLA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LYPLA1; Polyclonal Antibody; ID (LYPLA1; APT1; LPL1; Acyl-protein thioesterase 1; Lysophospholipase 1; Lysophospholipase I) (PE); Lysophospholipase I; anti-LYPLA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LYPLA1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LYPLA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the Central region of human LYPLA1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LYPLA1 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the LYPLA1 antibody detected the LYPLA1 protein (arrow).)

Related Product Information for anti-LYPLA1 antibody

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. The use of alternate polyadenylation sites has been found for this gene. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet.

Product Categories/Family for anti-LYPLA1 antibody

Antibodies; Enzymes

References

Satou, M., et al. Endocrinology 151(10):4765-4775(2010). Dekker, F.J., et al. Nat. Chem. Biol. 6(6):449-456(2010). Hirano, T., et al. Biochim. Biophys. Acta 1791(8):797-805(2009). Siegel, G., et al. Nat. Cell Biol. 11(6):705-716(2009). Devedjiev, Y., et al. Structure 8(11):1137-1146(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 5453722
• NCBI GeneID: 10434
• NCBI Accession #: NP_006321.1
• NCBI GenBank Nucleotide #: NM_006330.2
• UniProt Accession #: O75608; O43202; Q9UQF9
• Molecular Weight: 24,670 Da
• NCBI Official Full Name: acyl-protein thioesterase 1
• NCBI Official Synonym Full Names: lysophospholipase I
• NCBI Official Symbol: LYPLA1
• NCBI Official Synonym Symbols: LPL1; APT-1; hAPT1; LYSOPLA
• NCBI Protein Information: acyl-protein thioesterase 1; LPL-I; lysoPLA I; lysophospholipase 1; acyl-protein thioesterase-1; lysophospholipid-specific lysophospholipase
• UniProt Protein Name: Acyl-protein thioesterase 1
• UniProt Gene Name: LYPLA1
• UniProt Synonym Gene Names: APT1; LPL1; APT-1; hAPT1; LPL-I; LysoPLA I
• UniProt Entry Name: LYPA1_HUMAN

NCBI Description

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. The use of alternate polyadenylation sites has been found for this gene. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq, Jul 2008]

Uniprot Description

LYPLA1: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity and also low lysophospholipase activity. Belongs to the AB hydrolase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Phospholipase; EC 3.1.2.-

Chromosomal Location of Human Ortholog: 8q11.23

Cellular Component: mitochondrion; cytosol

Molecular Function: palmitoyl-(protein) hydrolase activity; lysophospholipase activity

Biological Process: negative regulation of Golgi to plasma membrane protein transport; regulation of nitric-oxide synthase activity; fatty acid metabolic process; nitric oxide metabolic process; protein depalmitoylation