Mouse anti-Human LYN Monoclonal Antibody | anti-LYN antibody

LYN Antibody

Cat. Num. AAA9200211

• Gene Names: LYN; JTK8; p53Lyn; p56Lyn
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: LYN; Monoclonal Antibody; LYN Antibody; Tyrosine-protein kinase Lyn; Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn; JTK8; anti-LYN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Clone Number: 1174CT22.3.1.1
• Specificity: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-210 amino acids from human.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 491

• Applicable Applications for anti-LYN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Recombinant Protein
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from A431, Raji cell line (from left to right), using LYN Antibody. MBS9200211 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-LYN antibody

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down- regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down- regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3- kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr- 72'.

Product Categories/Family for anti-LYN antibody

Signal Transduction

References

Yamanashi Y.,et al.Mol. Cell. Biol. 7:237-243(1987).
Rider L.G.,et al.Gene 138:219-222(1994).
Partanen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990).
Bielke W.,et al.Biochem. Biophys. Res. Commun. 186:1403-1409(1992).
Roifman C.M.,et al.Biochem. Biophys. Res. Commun. 194:222-225(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 162287326
• NCBI GeneID: 4067
• NCBI Accession #: NP_001104567.1
• NCBI GenBank Nucleotide #: NM_001111097.2
• UniProt Accession #: P07948; A0AVQ5
• Molecular Weight: 58574
• NCBI Official Full Name: tyrosine-protein kinase Lyn isoform B
• NCBI Official Synonym Full Names: LYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: LYN
• NCBI Official Synonym Symbols: JTK8; p53Lyn; p56Lyn
• NCBI Protein Information: tyrosine-protein kinase Lyn
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LYN
• UniProt Synonym Gene Names: JTK8
• UniProt Entry Name: LYN_HUMAN

NCBI Description

This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: Golgi apparatus; mitochondrial crista; extrinsic to internal side of plasma membrane; mast cell granule; perinuclear region of cytoplasm; cytoplasm; postsynaptic density; plasma membrane; mitochondrial intermembrane space; cytosol; nucleus; lipid raft

Molecular Function: integrin binding; protein binding; enzyme binding; ubiquitin protein ligase binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; platelet-derived growth factor receptor binding; phosphoprotein binding; receptor signaling protein tyrosine kinase activity; glycosphingolipid binding; SH3 domain binding; ATP binding; receptor binding

Biological Process: regulation of mast cell degranulation; negative regulation of MAP kinase activity; central nervous system development; viral reproduction; response to toxin; positive regulation of B cell receptor signaling pathway; protein amino acid phosphorylation; response to organic cyclic substance; response to carbohydrate stimulus; regulation of B cell apoptosis; B cell receptor signaling pathway; erythrocyte differentiation; oligodendrocyte development; tolerance induction to self antigen; response to drug; platelet activation; negative regulation of immune response; response to sterol depletion; immune response-regulating cell surface receptor signaling pathway; response to amino acid stimulus; regulation of erythrocyte differentiation; histamine secretion by mast cell; T cell costimulation; cytokine secretion; leukocyte migration; regulation of mast cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; axon guidance; regulation of cytokine secretion; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; response to hormone stimulus; negative regulation of B cell proliferation; signal transduction; regulation of cytokine production; response to insulin stimulus; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; platelet degranulation; B cell homeostasis; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; ephrin receptor signaling pathway; Fc receptor mediated inhibitory signaling pathway; response to axon injury; Fc receptor mediated stimulatory signaling pathway; inflammatory response; regulation of B cell receptor signaling pathway; regulation of protein amino acid phosphorylation; negative regulation of myeloid leukocyte differentiation; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell motility; regulation of release of sequestered calcium ion into cytosol; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of toll-like receptor 4 signaling pathway; regulation of inflammatory response; cellular response to extracellular stimulus; innate immune response; blood coagulation; response to DNA damage stimulus; positive regulation of cell migration