Rabbit Lyn Polyclonal Antibody | anti-LYN antibody

Lyn, phosphorylated (Tyr507) (p53/56lyn)

Cat. Num. AAA630844

• Gene Names: LYN; JTK8; p53Lyn; p56Lyn
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Lyn; Polyclonal Antibody; phosphorylated (Tyr507) (p53/56lyn); Anti -Lyn; anti-LYN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of human Lyn phosphorylated at tyrosine 507. Species Crossreactivity: Predicted to crossreact with mouse and rat based on sequence homology.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-LYN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr507 of human Lyn.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12ug/ml for 2 minutes) or untreated, using Phospho-Lyn (Tyr507) Antibody. The phospho-specificity of the antibody was characterized by treating the membrane without or with calf intestinal alkaline phosphatase (CIP) after Western transfer.)

Related Product Information for anti-LYN antibody

Lyn is a member of the src family of tyrosine kinases responsible for the regulation of cell signaling mechanisms. Lyn's function has been studied in cells of hematopoetic origin where it is associated with the high af nity IgE receptor in basophils and with the p120 Ras-GAP in thrombin-stimulated platelets. Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosyl residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family: autophosphorylation of tyrosine 396 (equivalent to tyrosine 416 of c-Src) located in the catalytic domain correlates with enzyme activation, while Csk-mediated phosphorylation of the C-terminal tyrosine 507 (equivalent to tyrosine 527 of c-Src) inactives the kinases. Lyn was tyrosine phosphorylated and activated upon association with cell surface receptors such as the B cell Ag recepter (BCR) and CD40 (2-4). Knockout mice have shown that the net effect of Lyn deficiency is to render B cells hyperactive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the downmodulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

Product Categories/Family for anti-LYN antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 307144
• NCBI GeneID: 4067
• UniProt Accession #: P07948; A0AVQ5
• Molecular Weight: 58,574 Da
• NCBI Official Full Name: lyn tyrosine kinase
• NCBI Official Synonym Full Names: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• NCBI Official Symbol: LYN
• NCBI Official Synonym Symbols: JTK8; p53Lyn; p56Lyn
• NCBI Protein Information: tyrosine-protein kinase Lyn; lck/Yes-related novel protein tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LYN
• UniProt Synonym Gene Names: JTK8
• UniProt Entry Name: LYN_HUMAN

NCBI Description

This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Protein kinase, tyrosine (non-receptor); Oncoprotein; Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: Golgi apparatus; mitochondrial crista; extrinsic to internal side of plasma membrane; mast cell granule; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; cytosol; lipid raft

Molecular Function: integrin binding; protein binding; enzyme binding; ubiquitin protein ligase binding; protein-tyrosine kinase activity; platelet-derived growth factor receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; receptor signaling protein tyrosine kinase activity; glycosphingolipid binding; SH3 domain binding; ATP binding; receptor binding

Biological Process: regulation of mast cell degranulation; negative regulation of MAP kinase activity; central nervous system development; viral reproduction; response to toxin; positive regulation of B cell receptor signaling pathway; protein amino acid phosphorylation; response to organic cyclic substance; response to carbohydrate stimulus; regulation of B cell apoptosis; B cell receptor signaling pathway; erythrocyte differentiation; oligodendrocyte development; tolerance induction to self antigen; response to drug; platelet activation; negative regulation of immune response; response to sterol depletion; immune response-regulating cell surface receptor signaling pathway; response to amino acid stimulus; regulation of erythrocyte differentiation; histamine secretion by mast cell; T cell costimulation; cytokine secretion; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of mast cell activation; axon guidance; regulation of cytokine secretion; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; response to hormone stimulus; negative regulation of B cell proliferation; signal transduction; response to insulin stimulus; regulation of cytokine production; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; platelet degranulation; B cell homeostasis; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; ephrin receptor signaling pathway; Fc receptor mediated inhibitory signaling pathway; response to axon injury; Fc receptor mediated stimulatory signaling pathway; inflammatory response; regulation of B cell receptor signaling pathway; regulation of protein amino acid phosphorylation; negative regulation of myeloid leukocyte differentiation; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell motility; regulation of release of sequestered calcium ion into cytosol; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of toll-like receptor 4 signaling pathway; regulation of inflammatory response; cellular response to extracellular stimulus; innate immune response; blood coagulation; response to DNA damage stimulus; positive regulation of cell migration