Rabbit anti-Human LUM Polyclonal Antibody | anti-LUM antibody

LUM (Lumican, LDC, Keratan Sulfate Proteoglycan Lumican, KSPG Lumican, SLRR2D) (PE)

Cat. Num. AAA6485560

• Gene Names: LUM; LDC; SLRR2D
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: LUM; Polyclonal Antibody; LUM (Lumican; LDC; Keratan Sulfate Proteoglycan Lumican; KSPG Lumican; SLRR2D) (PE); anti-LUM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LUM.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LUM antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; ELISA: 1:1000; WB: 1:50-1:100; IHC-P: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion of amino acids within aa205-235 from the center region of human LUM, conjugated to KLH.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of LUM antibody (Center) in A375 cell line lysates (35ug/lane). LUM (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with LUM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LUM Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-LUM antibody

Antibodies; Extracellular Matrix Proteins

References

1. Wang, P., Li, S. Invest. Ophthalmol. Vis. Sci. 50(4), 1546-1551 (2009). 2. Matsuda, Y., Yamamoto, T. Int. J. Oncol. 33(6), 1177-1185 (2008). 3. Kelemen, L.E., Couch, F.J. Breast Cancer Res. 10(6), R98 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4505047
• NCBI GeneID: 4060
• NCBI Accession #: NP_002336
• NCBI GenBank Nucleotide #: NM_002345.3
• UniProt Accession #: P51884; Q96QM7; B2R6R5
• Molecular Weight: 39kDa (343aa)
• NCBI Official Full Name: lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: LUM
• NCBI Official Synonym Symbols: LDC; SLRR2D
• NCBI Protein Information: lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: LUM
• UniProt Synonym Gene Names: LDC; SLRR2D; KSPG lumican
• UniProt Entry Name: LUM_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; proteinaceous extracellular matrix; fibrillar collagen; Golgi lumen; extracellular region

Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; glycosaminoglycan metabolic process; visual perception; cartilage development; keratan sulfate biosynthetic process; carbohydrate metabolic process; positive regulation of transcription from RNA polymerase II promoter; pathogenesis; keratan sulfate catabolic process; response to organic cyclic substance