Rabbit anti-Human Kallikrein 6 Polyclonal Antibody | anti-KLK1 antibody

Kallikrein 6, ID (KLK6, PRSS18, PRSS9, Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme)

Cat. Num. AAA649453

• Gene Names: KLK1; hK1; KLKR; Klk6
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: Kallikrein 6; Polyclonal Antibody; ID (KLK6; PRSS18; PRSS9; Kallikrein-6; Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme); Anti -Kallikrein 6; anti-KLK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-KLK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: Kallikrein 6 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134~164 amino acids from the central region of human KLK6.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLK6 (arrow) using rabbit polyclonal KLK6 Antibody (L141) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KLK6 gene.)

Immunohistochemistry (IHC)

(Kallikrein 6(KLK6) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Kallikrein 6(KLK6) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Kallikrein 6 (KLK6) Antibody (Center) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KLK1 antibody

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The KLK6 enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease.

Product Categories/Family for anti-KLK1 antibody

Antibodies; Abs to Kallikrein

NCBI and Uniprot Product Information

• NCBI GI #: 386843
• NCBI GeneID: 3816
• UniProt Accession #: P06870; Q66US9; Q86U61; Q8TCV8; Q9BS53; Q9NQU4; Q9UD19; Q9UMJ1
• Molecular Weight: 28,890 Da
• NCBI Official Full Name: kallikrein, partial
• NCBI Official Synonym Full Names: kallikrein 1
• NCBI Official Symbol: KLK1
• NCBI Official Synonym Symbols: hK1; KLKR; Klk6
• NCBI Protein Information: kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein
• UniProt Protein Name: Kallikrein-1
• Protein Family: Blarinasin
• UniProt Gene Name: KLK1
• UniProt Entry Name: KLK1_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Tissue specificity: Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. Ref.4

Post-translational modification: The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In Ref.13, GalNAc was detected with the corresponding peptides but not located.

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.Contains 1 peptidase S1 domain.