Rabbit anti-Human LSM14A Polyclonal Antibody | anti-LSM14A antibody

Anti-LSM14A Antibody, Rabbit Polyclonal

Cat. Num. AAA8102051

• Gene Names: LSM14A; RAP55; FAM61A; RAP55A; C19orf13
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: LSM14A; Polyclonal Antibody; Anti-LSM14A Antibody; Rabbit Polyclonal; LSM14A Antibody; Rabbit PAb; Antigen Affinity Purified; SCD6 homolog A (S. cerevisiae); Anti-C19orf13 Antibody; Anti-FAM61A Antibody; Anti-RAP55 Antibody; Anti-RAP55A Antibody; anti-LSM14A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LSM14A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-LSM14A antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human LSM14A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human LSM14A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-LSM14A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:57 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human LSM14A in human tonsil with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human LSM14A in human skin with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-LSM14A antibody

Human LSm14A has recently been found as a processing body-associated sensor of intracellular viral nucleic acids and triggers signaling for type I IFN expression. Human LSm14A is a key component of the processing body (P-body) assembly that mediates interferon-beta (IFN-beta) production by sensing viral RNA or DNA. LSm14A, a member of the LSM family involved in RNA processing in the processing bodies, binds to synthetic or viral RNA and DNA and mediates IRF3 activation and IFN-beta induction. LSm14A is a key innate immunity component of the processing body (P-body) that mediates interferon-beta (IFN-beta) signaling by viral RNA. Knockdown of LSm14A inhibits cytosolic RNA-and DNA-trigger type I IFN production and cellular antiviral response. Moreover, LSm14A is essential for early-phase induction of IFN-beta after either RNA or DNA virus infection. chicken LSm14A (cLSm14A) is an important sensor that mediates innate immunity in the chicken against NDV infections.

References

Wen G, et al. (2013) Functional characterization of porcine lsm14a in ifn-beta induction. Vet Immunol Immunopathol 155 (1-2): 110-116.Tian L, et al. (2019) Transcriptional responses of lsm14a after infection of blue eggshell layers with newcastle disease viruses. J Vet Med Sci 81 (10): 1468-1474.Li Y, et al. (2012) Lsm14a is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection. Proc Natl Acad Sci U S A 109 (29): 11770-11775.Hua K, et al. (2017) Functional characterization of duck lsm14a in ifn-beta induction. Dev Comp Immunol 76 255-261.

NCBI and Uniprot Product Information

• NCBI GI #: 71648673
• NCBI GeneID: 26065
• UniProt Accession #: Q8ND56; Q76LX7; Q96AR3; Q96K73; Q96SN5; Q9UFR3; B4DTG6
• Molecular Weight: 463
• NCBI Official Full Name: Protein LSM14 homolog A
• NCBI Official Synonym Full Names: LSM14A, SCD6 homolog A (S. cerevisiae)
• NCBI Official Symbol: LSM14A
• NCBI Official Synonym Symbols: RAP55; FAM61A; RAP55A; C19orf13
• NCBI Protein Information: protein LSM14 homolog A; hRAP55; hRAP55A; alphaSNBP; protein SCD6 homolog; RNA-associated protein 55; RNA-associated protein 55A; putative alpha-synuclein-binding protein; family with sequence similarity 61, member A
• UniProt Protein Name: Protein LSM14 homolog A
• Protein Family: Protein
• UniProt Gene Name: LSM14A
• UniProt Synonym Gene Names: C19orf13; FAM61A; RAP55; RAP55A; AlphaSNBP; hRAP55; hRAP55A
• UniProt Entry Name: LS14A_HUMAN

NCBI Description

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008]

Uniprot Description

alphaSNBP(A): Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs. Belongs to the LSM14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: intracellular membrane-bound organelle; stress granule; cytoplasm

Molecular Function: single-stranded RNA binding; double-stranded DNA binding; double-stranded RNA binding

Biological Process: regulation of translation; multicellular organismal development; cytoplasmic mRNA processing body assembly