Rabbit LRSAM1 Polyclonal Antibody | anti-LRSAM1 antibody

LRSAM1 Antibody

Cat. Num. AAA9609797

• Gene Names: LRSAM1; TAL; CMT2P; RIFLE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: LRSAM1; Polyclonal Antibody; LRSAM1 Antibody; CMT2P; E3 ubiquitin protein ligase LRSAM1; E3 ubiquitin-protein ligase LRSAM1; EC 6.3.2.-; FLJ31641; hTAL; Leucine rich repeat and sterile alpha motif containing 1; Leucine rich repeat and sterile alpha motif containing protein 1; Leucine-rich repeat and sterile alpha motif-containing protein 1; LRSM1_HUMAN; OTTHUMP00000022174; OTTHUMP00000022175; RIFLE; TAL; Tsg101 associated ligase; Tsg101-associated ligase; anti-LRSAM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LRSAM1 antibody detects endogenous levels of total LRSAM1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 723

• Applicable Applications for anti-LRSAM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human LRSAM1
• Subcellular Location: Cytoplasm. Displays a punctuate distribution and localizes to a submembranal ring.
• Tissue Specificity: Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Dog (91%), Chicken (90%), Xenopus (91%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from rat muscle, using LRSAM1 Antibody.)

Western Blot (WB)

(Western blot analysis of LRSAM1 using HT29 whole cell lysates)

Related Product Information for anti-LRSAM1 antibody

Function: E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos (PubMed:15256501). Bacterial recognition protein that defends the cytoplasm from invasive pathogens (PubMed:23245322). Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy) (PubMed:23245322, PubMed:25484098).
Subunit Structure: Interacts with TSG101 (PubMed:17556548). Interacts with PHF23 (PubMed:25484098). Interacts with FUS (PubMed:27615052).
Post-translational Modifications: Ubiquitination promoted by PHF23 leads to proteasomal degradation.
Similarity: The coiled coil domains interact with the SB domain of TSG101.The PTAP motifs mediate the binding to UEV domains.The LRR domain is necessary and sufficient for localization to bacterial targets.The RING domain is required for ubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 53729361
• NCBI GeneID: 90678
• NCBI Accession #: NP_001005373.1
• NCBI GenBank Nucleotide #: NM_001005373.3
• UniProt Accession #: Q6UWE0; Q5VVV0; Q8NB40; Q96GT5; Q96MX5; Q96MZ7
• Molecular Weight: Observed: 84 kDa; Predicted: 84 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase LRSAM1 isoform 1
• NCBI Official Synonym Full Names: leucine rich repeat and sterile alpha motif containing 1
• NCBI Official Symbol: LRSAM1
• NCBI Official Synonym Symbols: TAL; CMT2P; RIFLE
• NCBI Protein Information: E3 ubiquitin-protein ligase LRSAM1
• UniProt Protein Name: E3 ubiquitin-protein ligase LRSAM1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: LRSAM1
• UniProt Synonym Gene Names: TAL1 PublicationManual assertion based on opinion iniRef.5; hTAL1 PublicationManual assertion based on opinion iniRef.5

NCBI Description

This gene encodes a ring finger protein involved in a variety of functions, including regulation of signaling pathways and cell adhesion, mediation of self-ubiquitylation, and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos (PubMed:15256501). Bacterial recognition protein that defends the cytoplasm from invasive pathogens (PubMed:23245322). Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy) (PubMed:23245322, PubMed:25484098).