Rabbit anti-Human LRRC8A Polyclonal Antibody | anti-LRRC8A antibody

LRRC8A, CT (LRRC8A, KIAA1437, LRRC8, Leucine-rich repeat-containing protein 8A) (APC)

Cat. Num. AAA6317297

• Gene Names: LRRC8A; AGM5; LRRC8; SWELL1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LRRC8A; Polyclonal Antibody; CT (LRRC8A; KIAA1437; LRRC8; Leucine-rich repeat-containing protein 8A) (APC); Leucine-rich repeat-containing protein 8A; anti-LRRC8A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 810

• Applicable Applications for anti-LRRC8A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LRRC8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782-810 amino acids from the C-terminal region of human LRRC8A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LRRC8A Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the LRRC8A antibody detected the LRRC8A protein (arrow).)

Related Product Information for anti-LRRC8A antibody

This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Product Categories/Family for anti-LRRC8A antibody

Antibodies; Leucine-rich Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Smits, G., et al. Mol. Immunol. 41(5):561-562(2004). Kubota, K., et al. FEBS Lett. 564 (1-2), 147-152 (2004): Sawada, A., et al. J. Clin. Invest. 112(11):1707-1713(2003). Conley, M.E. J. Clin. Invest. 112(11):1636-1638(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 62241040
• NCBI GeneID: 56262
• NCBI Accession #: NP_062540.2
• NCBI GenBank Nucleotide #: NP_062540.2
• UniProt Accession #: Q8IWT6
• NCBI Official Full Name: volume-regulated anion channel subunit LRRC8A
• NCBI Official Synonym Full Names: leucine rich repeat containing 8 VRAC subunit A
• NCBI Official Symbol: LRRC8A
• NCBI Official Synonym Symbols: AGM5; LRRC8; SWELL1
• NCBI Protein Information: volume-regulated anion channel subunit LRRC8A
• UniProt Protein Name: Volume-regulated anion channel subunit LRRC8A
• Protein Family: Volume-regulated anion channel
• UniProt Gene Name: LRRC8A
• UniProt Synonym Gene Names: KIAA1437; LRRC8; SWELL1
• UniProt Entry Name: LRC8A_HUMAN

NCBI Description

This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRC8A: Involved in B-cell development. Required for the pro-B cell to pre-B cell transition. Defects in LRRC8A are the cause of agammaglobulinemia type 5 (AGM5). It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. A chromosomal aberration involving LRRC8 has been found in a patient with congenital agammaglobulinemia. Translocation t(9;20)(q33.2;q12). The translocation truncates the LRRC8 gene, resulting in deletion of the eighth, ninth, and half of the seventh LRR domains.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cell surface; membrane; plasma membrane

Molecular Function: anion channel activity; protein binding; volume-sensitive anion channel activity

Biological Process: anion transport; cell volume homeostasis; pre-B cell differentiation; response to osmotic stress

Disease: Agammaglobulinemia 5, Autosomal Dominant