Rabbit anti-Human LRP2 Polyclonal Antibody | anti-LRP2 antibody

LRP2, CT (Low-density Lipoprotein Receptor-related Protein 2, Megalin, Glycoprotein 330, gp330) (FITC)

Cat. Num. AAA6485227

• Gene Names: LRP2; DBS; GP330; LRP-2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: LRP2; Polyclonal Antibody; CT (Low-density Lipoprotein Receptor-related Protein 2; Megalin; Glycoprotein 330; gp330) (FITC); anti-LRP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LRP2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 4655

• Applicable Applications for anti-LRP2 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human LRP2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-LRP2 antibody

Antibodies; Receptors

References

1. Christensen, E.I., et al., Nat. Rev. Mol. Cell Biol. 3(4):256-266 (2002). 2. Hjalm, G., et al., Eur. J. Biochem. 239(1):132-137 (1996). 3. Farquhar, M.G., et al., J. Am. Soc. Nephrol. 6(1):35-47 (1995). 4. Kounnas, M.Z., et al., J. Biol. Chem. 270(22):13070-13075 (1995). 5. Korenberg, J.R., et al., Genomics 22(1):88-93 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 126012573
• NCBI GeneID: 4036
• NCBI Accession #: NP_004516
• NCBI GenBank Nucleotide #: NP_004516.2
• UniProt Accession #: P98164
• Molecular Weight: 521.919
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 2
• NCBI Official Synonym Full Names: LDL receptor related protein 2
• NCBI Official Symbol: LRP2
• NCBI Official Synonym Symbols: DBS; GP330; LRP-2
• NCBI Protein Information: low-density lipoprotein receptor-related protein 2
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 2
• Protein Family: LRP2-binding protein
• UniProt Gene Name: LRP2
• UniProt Synonym Gene Names: LRP-2; gp330
• UniProt Entry Name: LRP2_HUMAN

NCBI Description

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

Uniprot Description

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi apparatus; endoplasmic reticulum; brush border membrane; endocytic vesicle; lysosomal membrane; lysosome; apical plasma membrane; integral to membrane; plasma membrane; coated pit; endosome; receptor complex

Molecular Function: protein binding; calcium ion binding; SH3 domain binding

Biological Process: steroid metabolic process; receptor-mediated endocytosis; cell proliferation; phototransduction, visible light; forebrain development; protein amino acid glycosylation; endocytosis; lipid metabolic process; retinoid metabolic process; vitamin D metabolic process

Disease: Donnai-barrow Syndrome