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Western Blot (WB) (LPPR4 Antibody (C-term) western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).This demonstrates the LPPR4 antibody detected the LPPR4 protein (arrow).)

Rabbit anti-Human LPPR4 Polyclonal Antibody | anti-LPPR4 antibody

LPPR4, CT (LPPR4, KIAA0455, PRG1, Lipid phosphate phosphatase-related protein type 4, Brain-specific phosphatidic acid phosphatase-like protein 1, Plasticity-related gene 1 protein)

Gene Names
LPPR4; LPR4; PHP1; PRG1; PRG-1; RP4-788L13.1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
LPPR4; Polyclonal Antibody; CT (LPPR4; KIAA0455; PRG1; Lipid phosphate phosphatase-related protein type 4; Brain-specific phosphatidic acid phosphatase-like protein 1; Plasticity-related gene 1 protein); Anti -LPPR4; anti-LPPR4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-LPPR4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
LPPR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 675-705 from the C-terminal region of human LPPR4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LPPR4 Antibody (C-term) western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).This demonstrates the LPPR4 antibody detected the LPPR4 protein (arrow).)

Western Blot (WB) (LPPR4 Antibody (C-term) western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).This demonstrates the LPPR4 antibody detected the LPPR4 protein (arrow).)

Immunohistochemistry (IHC)

(LPPR4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LPPR4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (LPPR4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LPPR4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LPPR4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (LPPR4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LPPR4 antibody
LPPR4 belongs to the lipid phosphate phosphatase (LPP) family. LPPs catalyze the dephosphorylation of a number of bioactive lipid mediators that regulate a variety of cell functions. This protein is specifically expressed in neurons. It is located in the membranes of outgrowing axons and has been shown to be important for axonal outgrowth during development and regenerative sprouting.
Product Categories/Family for anti-LPPR4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,983 Da
NCBI Official Full Name
lipid phosphate phosphatase-related protein type 4 isoform 2
NCBI Official Symbol
LPPR4
NCBI Official Synonym Symbols
LPR4; PHP1; PRG1; PRG-1; RP4-788L13.1
NCBI Protein Information
lipid phosphate phosphatase-related protein type 4; plasticity related gene 1; plasticity-related gene 1 protein; brain-specific phosphatidic acid phosphatase-like protein 1
UniProt Protein Name
Lipid phosphate phosphatase-related protein type 4
UniProt Gene Name
LPPR4
UniProt Synonym Gene Names
KIAA0455; PRG1; PRG-1
UniProt Entry Name
LPPR4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the lipid phosphate phosphatase (LPP) family. LPPs catalyze the dephosphorylation of a number of bioactive lipid mediators that regulate a variety of cell functions. This protein is specifically expressed in neurons. It is located in the membranes of outgrowing axons and has been shown to be important for axonal outgrowth during development and regenerative sprouting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

LPPR4: Hydrolyzes lysophosphatidic acid (LPA). Facilitates axonal outgrowth during development and regenerative sprouting. In the outgrowing axons acts as an ecto-enzyme and attenuates phospholipid-induced axon collapse in neurons and facilitates outgrowth in the hippocampus. Belongs to the PA-phosphatase related phosphoesterase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Phosphatase, lipid; Cell development/differentiation; EC 3.1.3.4; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p21.2

Cellular Component: integral to plasma membrane

Molecular Function: phosphatidate phosphatase activity; lipid phosphatase activity

Biological Process: axonogenesis; inner ear development; phospholipid dephosphorylation

Research Articles on LPPR4

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Product Notes

The LPPR4 lppr4 (Catalog #AAA6009225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LPPR4, CT (LPPR4, KIAA0455, PRG1, Lipid phosphate phosphatase-related protein type 4, Brain-specific phosphatidic acid phosphatase-like protein 1, Plasticity-related gene 1 protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LPPR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the LPPR4 lppr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LPPR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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